A novel method to crystallizing proteins by using two-dimensional organic-inorganichybrid material

2014 Fiscal Year Final Research Report

• Project/Area Number: 24657068
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: YAO Min 北海道大学, 先端生命科学研究科(研究院), 教授 (40311518)
• Research Collaborator: YAO Ken 中国上海大学, 理学院, 教授
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: タンパク質結晶化 / 規則的二次元分子 / CPAPhS / 結晶核形成 / X線結晶構造解析 / 分子材料

Outline of Final Research Achievements

In order to increase the probability of forming crystal kernels for proteins, we develop a novel method by using chemical material. In this method, we use a new material which is regular and functional two-dimensional organic-inorganic hybrid material. We have got succeeded to bind peptides on it in regular arrangement and link them to proteins. We tried to crystallize Trypsin and Rpf2-Rrs1 complex by using this new material with regular peptides, and found a way to develop different new materials further.

Free Research Field

構造生物学，タンパク質結晶学