• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Development of Novel monitoring method of autophagy

Research Project

  • PDF
Project/Area Number 24657089
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionOsaka University

Principal Investigator

NODA Takeshi  大阪大学, 歯学研究科(研究院), 教授 (00290908)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsオートファジー
Research Abstract

Autophagy is a process that the cell degrades its own components and reutilize them. Several environmental stress such as starvation induces autophagy and proceeds gradually and finally it is terminated. As the method for monitoring autophagy, I previously developed ALP assay in the yeast and the methods is still widely used. ALP assay enables quantitative measurement of autophagy based on the enzymatic activity of alkaline phosphatase. This time, I tried to developed similar method to ALP assay in mammalian cells. I generated various version of acid phosphatase to test if their activity is correlated with the progression of autophagy, but finally successful one could not be obtained. On the other hand, we succeeded in the development of genome wide ALP assay.

  • Research Products

    (10 results)

All 2013 Other

All Journal Article (3 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin2013

    • Author(s)
      Fujita N, Morita E, (equally contributed) Itoh T, Tanaka A, Nakaoka M, Osada Y, Umemoto T, Saitoh T, Nakatogawa H, Kobayashi S, Haraguchi T, Guan JL, Iwai K, Tokunaga F, Saito K, Ishibashi K, Akira S, Fukuda M, Noda T, Yoshimori T
    • Journal Title

      The Journal of Cell Biology

      Volume: 203(1) Pages: 115-128

  • [Journal Article] Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury2013

    • Author(s)
      Maejima I, Takahashi A, Omori H, Kimura T, Takabatake Y, Saitoh T, Yamamoto A, Hamasaki M, Noda T, Isaka Y, Yoshimori T
    • Journal Title

      EMBO journal

      Pages: 2336-2347

  • [Journal Article] TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy

    • Author(s)
      Shirahama-Noda K, Kira S, Yoshimori T, Noda T
    • Journal Title

      Journal of Cell Science

      Volume: 126 Pages: 4963-4973

  • [Presentation] TRAPPIII is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy2013

    • Author(s)
      野田健司
    • Organizer
      第7回オートファジー研究会
    • Place of Presentation
      掛川
    • Year and Date
      20131219-21
  • [Presentation] Regulatory Mechanism of Autophagy in yeast2013

    • Author(s)
      T NODA
    • Organizer
      3rd China-Japan symposium on autophagy
    • Place of Presentation
      Dunhuang China
    • Year and Date
      20131014-18
  • [Presentation] TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy2013

    • Author(s)
      Shirahama-Noda K, Kira S, Yoshimori T, Noda T
    • Organizer
      Gordon research conference molecular membrane biology
    • Place of Presentation
      アメリカニューハンプシャーProctor Academy Andover
    • Year and Date
      20130700
  • [Presentation] 長時間飢餓条件下におけるオートファジー停止機構の研究2013

    • Author(s)
      野口雅史、吉良新太郎、吉森保、野田健司
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-03
  • [Presentation] オートファジーとTORC1キナーゼの制御機構におけるNpr2の役割2013

    • Author(s)
      吉良新太郎、田端桂介、吉森保、野田健司
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2013-06-19
  • [Presentation] Critical role of PtdIns3P turn over in autophagy regulation2013

    • Author(s)
      T NODA
    • Organizer
      The 90^<th> Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Tower Hall Funahori, Tokyo
    • Year and Date
      2013-03-29
  • [Remarks]

    • URL

      http://web.dent.osaka-u.ac.jp/~cfos/index.html

URL: 

Published: 2015-06-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi