• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Study on structure-function relationship of KcsA channel by single molecule manipulation.

Research Project

  • PDF
Project/Area Number 24657110
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionOkayama University (2013)
The Graduate School for the Creation of New Photonics Industries (2012)

Principal Investigator

IDE Toru  岡山大学, 自然科学研究科, 教授 (60231148)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsイオンチャネル / 1分子計測 / 単一チャネル電流
Research Abstract

Our purpose was to develop an apparatus for simulataneous mechanical manipulation and optical recording of single ion channel protein, KcsA, by which we measure inter- and intra-molecule interactions that activate the channel. We have succeeded in (1)single molecule manipulation of KcsA immobilized at the tip of a fine glass needle (2)developing a technique for mechanically incorporating the KcsA into an artificial bilayer (3)making the measurement efficiency much higher than conventional methods by optimizing experimental conditions.

  • Research Products

    (12 results)

All 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Automated Parallel Recordings of Topologically Identified Single Ion Channels2013

    • Author(s)
      Kawano R, Tsuji Y, Sato K, Osaki T, Kamiya K, Hirano M, Ide T, Miki N, Takeuchi S
    • Journal Title

      Scientific Reports

      Volume: 3 Pages: 1955

    • Peer Reviewed
  • [Journal Article] Uncovering the Protein Translocon at the Chloroplast Inner Envelope Membrane2013

    • Author(s)
      S. Kikuchi, J. Bédard, M. Hirano, Y. Hirabayashi, M. Oishi, M. Imai, M. Takase, T. Ide, M. Nakai
    • Journal Title

      Science

      Volume: 339(6119) Pages: 571-574

    • DOI

      10.1126/science.1229262

    • Peer Reviewed
  • [Journal Article] 「ちから」でイオンチャネルの機能を制御する2012

    • Author(s)
      井出徹、平野美奈子、奥野大地
    • Journal Title

      生物物理学会誌

      Volume: 52(6) Pages: 289-290

    • URL

      https://www.jstage.jst.go.jp/article/biophys/52/6/52_289/_pdf

  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2013

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      Biophysical Society 57th Annual Meeting
    • Place of Presentation
      フィラデルフィア(アメリカ合衆国)
    • Year and Date
      20130202-06
  • [Presentation] Coordination between the cytoplasmic domain and the inactivation gate in the KcsA channel2013

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2013-10-30
  • [Presentation] Reconstitution of ion channel immobilized on solid support into lipid bilayer2013

    • Author(s)
      D. Okuno, M. Hirano, Y. Onishi, T. Ide
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2013-10-30
  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2012

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      Molecular Science of Fluctuations toward Biological Functions, The 6th International Symposium
    • Place of Presentation
      京都
    • Year and Date
      20121205-06
  • [Presentation] Single molecule imaging of bio-molecules2012

    • Author(s)
      T. Ide, M. Hirano, D. Okuno
    • Organizer
      The 14th Takayanagi Kenjiro Memorial Symposium
    • Place of Presentation
      静岡
    • Year and Date
      2012-11-28
  • [Presentation] Reconstitution of ion channel into lipid bilayer using glass needle2012

    • Author(s)
      D. Okuno, M. Hirano, Y. Onishi, T. Yanagida, T. Ide
    • Organizer
      第50回日本生物物理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-24
  • [Presentation] Automated drug screening system for ion channel proteins using artificial cell membranes2012

    • Author(s)
      R. Kawano, Y. Tuji, K. Kimiya, T. Osaki, M. Hirano, T. Ide, N. Miki, S.Takeuchi
    • Organizer
      第50回日本生物物理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-24
  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2012

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      第50回日本生物物理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-23
  • [Remarks]

    • URL

      http://www.gpi.ac.jp/research/teacher/professor-05.html

URL: 

Published: 2015-06-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi