2013 Fiscal Year Final Research Report
Study on structure-function relationship of KcsA channel by single molecule manipulation.
| Project/Area Number |
24657110
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Okayama University (2013)
The Graduate School for the Creation of New Photonics Industries (2012) |
|---|
Principal Investigator |
IDE Toru 岡山大学, 自然科学研究科, 教授 (60231148)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | イオンチャネル / １分子計測 / 単一チャネル電流 |
|---|
| Research Abstract |
Our purpose was to develop an apparatus for simulataneous mechanical manipulation and optical recording of single ion channel protein, KcsA, by which we measure inter- and intra-molecule interactions that activate the channel. We have succeeded in (1)single molecule manipulation of KcsA immobilized at the tip of a fine glass needle (2)developing a technique for mechanically incorporating the KcsA into an artificial bilayer (3)making the measurement efficiency much higher than conventional methods by optimizing experimental conditions.
|
