The mechanism and functional significance of the nuclear translocation of metabolic enzymes.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657135
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YONEDA YOSHIHIRO 大阪大学, 生命機能研究科, 教授 (80191667)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 代謝酵素 / 核輸送 / importin / transaldolase

Research Abstract

In this study, we examined the mechanism and functional significance of the nuclear translocation of transaldolase. Our results show that transaldolase possesses a nuclear localization signal (NLS) at N-terminus, and is imported into the nucleus by multi-subfamily of importin-alpha in conjunction with importin-beta. Further, our analysis revealed that the overexpression of the nuclear or cytoplasmic transaldolase differentially affects the cellular metabolism.

Research Products

• [Journal Article] The role of Importin-βs in the maintenance and lineage commitment of mouse embryonic stem cells (2014)
  • Author(s): Sangel P, Oka M, Yoneda Y.
  • Journal Title: FEBS Open Bio Volume: 4 Pages: 112-120
  • DOI: 10.1016/j.fob.2014.01.001
  • Peer Reviewed
• [Journal Article] Importin alpha subtypes determine differential transcription factor localization in embryonic stem cells maintenance (2013)
  • Author(s): Yasuhara N, Yamagishi R, Arai Y, Mehmood R, Kimoto C, Fujita T, Touma K, Kaneko K, Kamikawa Y, Moriyama Y, Yanagida T, Kaneko H and Yoneda Y.
  • Journal Title: Dev. Cell Volume: 26 Pages: 123-135
  • DOI: 10.1016/j.devcel.2013.06.022
  • Peer Reviewed
• [Journal Article] Quantitative regulation of nuclear pore complex components by O-GlcNAcylation (2013)
  • Author(s): Mizuguchi-Hata C, Ogawa Y, Oka M and Yoneda Y.
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1833 Pages: 2682-2689
  • DOI: 10.1016/j.bbamcr.2013.06.008
  • Peer Reviewed
• [Journal Article] Cell density- dependent nuclear accumulation of ELK3 is involved in suppression of PAI-1 expression (2013)
  • Author(s): Tanaka S, Nakano K, Sekimoto T, Oka M and Yoneda Y.
  • Journal Title: Cell Struct. Funct Volume: 38 Pages: 145-154
  • DOI: 10.1247/csf.13007
  • Peer Reviewed
• [Journal Article] Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor 1 (2013)
  • Author(s): Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K and Ohkawa Y.
  • Journal Title: Nucleic Acids Res Volume: 41 Pages: 7060-7072
  • DOI: 10.1093/nar/gkt414
  • Peer Reviewed
• [Journal Article] Differential role for transcription factor Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells (2013)
  • Author(s): Oka M, Moriyama T, Asally M, Kawakami K and Yoneda Y.
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 15085-15097
  • DOI: 10.1074/jbc.M112.448837
  • Peer Reviewed
• [Journal Article] Improvement in protocol to generate homogeneous glutamatergic neurons from mouse embryonic stem cells reduced apoptosis. Biochem (2013)
  • Author(s): Fujiki R, Sato A, Hata K, Tashiro F, Yasuhara N, Miyazaki J, Yoneda Y, Fujitani M, Yamashita T.
  • Journal Title: Biophys. Res. Commun Volume: 430 Pages: 604-609
  • DOI: 10.1016/j.bbrc.2012.11.106
  • Peer Reviewed
• [Journal Article] The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza (2013)
  • Author(s): Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanabe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penniger JM, Arita M and Imai Y.
  • Journal Title: Cell Volume: 153 Pages: 112-125
  • DOI: 10.1016/j.cell.2013.02.027
  • Peer Reviewed
• [Journal Article] The Nuclear import factor importin alpha 4 can protect against oxidative stress. Biochim. Biophys (2013)
  • Author(s): Young JC, Ly-Huynh JD, Lescesen H, Miyamoto Y, Browne C, Yoneda Y, Koopman P, Loveland KL, Jans DA.
  • Journal Title: Acta-Molecular Cell Research Volume: 1833 Pages: 2348-2356
  • DOI: 10.1016/j.bbamcr.2013.06.007
  • Peer Reviewed
• [Journal Article] Downregulation of the small GTPase Ras-related nuclear protein accelerates cellular aging (2012)
  • Author(s): Nagai M and Yoneda Y.
  • Journal Title: Biochim. Biophys. Acta Volume: 1830 Pages: 2813-2819
  • DOI: 10.1016/j.bbagen.2012.11.001
  • Peer Reviewed