Microtubule-dependent dorsal determination in vertebrate embryogenesis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657152
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Nagoya University
• Principal Investigator: HIBI Masahiko 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 体軸形成 / ゼブラフィッシュ / 胚発生 / 遺伝学 / 微小管

Research Abstract

In amphibian and teleost embryos, dorsal determinants are initially localized to the vegetal pole and then transported to the dorsal side of the embryo along a microtubule array. The dorsal determinants activate the canonical Wnt pathway that is required for the formation of dorsal tissues. Using transgenic zebrafish lines expressing GFP-Tubulin and EB1-GFP, we found that a parallel microtubule array formed at the vegetal pole. The plus end of microtubule array was directed to the future dorsal side. The microtubules themselves moved to the future dorsal side and the vesicles moved along with vegetal microtubules. We found that Syntabulin, which is required for dorsal determination, surrounded vesicles and moved along with them to the dorsal side. Syntabulin interacted with Grip2a and Wntelss. Our data suggest that Syntabulin interacts with Grip2a and Wntless to recruit the vesicles containing the dorsal determinants onto the microtubules to transport them to the dorsal side.

Research Products

• [Journal Article] Sox5 functions as a fate switch in medaka pigment cell development (2014)
  • Author(s): Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H.
  • Journal Title: PLoS Genetics Volume: 10 Pages: e1004246
  • Peer Reviewed
• [Journal Article] The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish (2013)
  • Author(s): Kwon HB, Fukuhara S, Asakawa K, Ando K, Kashiwada T, Kawakami K, Hibi M, Kwon YG, Kim KW, Alitalo K, Mochizuki, N.
  • Journal Title: Development Volume: 140 Pages: 4081-4090
  • Peer Reviewed
• [Journal Article] Requirement of zebrafish ataxin-7 in differentiation of photoreceptors and cerebellar neurons (2012)
  • Author(s): Yanicostas C, Barbieri E, Hibi M, Brice A, Stevanin G, Soussi-Yanicostas N.
  • Journal Title: PLoS One Volume: 7 Pages: e250705
  • Peer Reviewed
• [Journal Article] Dynamic microtubules are the vegetal cortex predict the embryonic axis in zebrafish (2012)
  • Author(s): Tran LD, Hino H, Quach H, Lim S, Shindo A, Mimori-Kiyosue Y, Mione M, Ueno N, Winkler C, Hibi M, Sampath K.
  • Journal Title: Development Volume: 139 Pages: 3644-3652
  • Peer Reviewed
• [Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish (2012)
  • Author(s): Kuscha V, Fraser SL, Dias TB, Hibi M, Becker T, Becker CG.
  • Journal Title: J. Comp. Neurol Volume: 530 Pages: 3604-3616
  • Peer Reviewed
• [Presentation] Microtubule-mediated dorsal determination (2014)
  • Author(s): Hibi M, Hino H, Seki R, Shimizu T, Pelegri F.
  • Organizer: 6^<th> Asia Oceania Zebrafish Meeting
  • Place of Presentation: 香港、中国
  • Year and Date: 2014-01-21
  • Invited
• [Presentation] Involvement of microtubule-dependent transport of Wnt signaling components in zebrafish dorsal determination (2013)
  • Author(s): Hino H, Seki R, Shimizu T, Long TD, Sampath K, Hibi M.
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: 松江
  • Year and Date: 20130529-31
• [Presentation] Moving microtubule-mediated dorsal determination (2013)
  • Author(s): Hino H, Seki R, Shimizu T, Pelegri F, Hibi M.
  • Organizer: 第19回小型魚類研究会
  • Place of Presentation: 仙台
  • Year and Date: 2013-09-20
• [Presentation] Microtubule-Dependent Dorsal Determination in Zebrafish (2013)
  • Author(s): Hibi M.
  • Organizer: CDB Symposium 2013
  • Place of Presentation: 理化学研究所神戸
  • Year and Date: 2013-03-05
  • Invited
• [Presentation] Syntabulin-mediated transport of Wnt signaling machinery in zebrafish dorsal determination (2012)
  • Author(s): Seki R, Hino H, Takeda S, Pelegri F, Hibi M.
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
  • Year and Date: 20120922-23
• [Presentation] Formation of microtubule array for dorsal determination in zebrafish (2012)
  • Author(s): Hino H, Seki R, Long TD, Sampath K, Hibi M.
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
  • Year and Date: 20120922-23
• [Presentation] Microtubule-dependent dorsal determination in zebrafish (2012)
  • Author(s): Hino H, Seki R, Long TD, Sampath K, Shimizu T, Hibi M.
  • Organizer: 第45回日本発生生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20120528-31