Changing the period of the somite segmentation clock

2012 Fiscal Year Final Research Report

• Project/Area Number: 24657153
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: KAGEYAMA Ryoichiro 京都大学, ウイルス研究所, 教授 (80224369)
• Project Period (FY): 2012
• Keywords: イントロン / Hes7 / 発現振動 / 数理モデル / 体節 / 椎骨 / 自己抑制

Research Abstract

Periodic somite segmentation is controlled by the cyclic gene Hes7 whose oscillatory expression depends upon negative feedback with a delayed timing. Here, we show that reducing the number of introns within the Hes7 gene shortens the delay and results in a more rapid tempo of both Hes7 oscillation and somite segmentation, increasing the number of somites and vertebrae in the cervical and upper thoracic region. These results suggest that the number of introns is important for the appropriate tempo of the segmentation clock.

Research Products

• [Journal Article] Oscillatory links of Fgf signaling and Hes7 in the segmentation clock
  • Author(s): Harima, Y., and Kageyama, R
  • Journal Title: Curr. Opin. Genet. Dev Volume: (in press)
  • DOI: DOI:10.1016/j.gde.2013.02.005.
  • Peer Reviewed
• [Journal Article] Accelerating the tempo of the segmentation clock by reducing the number of introns in the Hes7 gene.
  • Author(s): Harima, Y., Takashima, Y., Ueda, Y., Ohtsuka, T., and Kageyama, R.
  • Journal Title: Cell Reports Volume: 3 Pages: 1-7
  • DOI: DOI:10.1016/i.celrer.2012.11.012.
  • Peer Reviewed
• [Presentation] Accelerated tempo of the segmentation clock bt reducing the number of introns in the Hes7 gene (2013)
  • Author(s): Harima, Y, Takashima, Y, Ueda, Y, Ohtsuka, T., and Kagevama. R
  • Organizer: CDB Symposium
  • Place of Presentation: 神戸
  • Year and Date: 2013-03-06
• [Presentation] Acceleratedtempo of the segmentation clock bt reducing the number of introns in the Hes7 gene (2012)
  • Author(s): Harima,Y,Takashima,Y,Ueda,Y,Ohtsuka,T,andKageyama.R
  • Organizer: Swiss Japanese Developmental Biology Meeting
  • Place of Presentation: Kyoto,11
  • Year and Date: 20121105-1108
• [Presentation] Accelerated tempo of the segmentation clock by reducing the number of introns in the Hes7 gene (2012)
  • Author(s): Harima, Y, Takashima, Y, Ueda, Y, Ohtsuka, T., and Kageyama, R
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] Hes oscillations in somitogenesis and neurogenesis (2012)
  • Author(s): Kageyama. R
  • Organizer: Swiss Japanese Developmental Biology Meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2012-11-08
• [Presentation] Ultradian oscillations in somitogenesis and neurogenesis (2012)
  • Author(s): Kageyama, R
  • Organizer: Department of Systems Biology Harvard Medical School Systems Biology Seminar Series
  • Place of Presentation: Boston, USA
  • Year and Date: 2012-10-11
• [Presentation] The significance of oscillatory gene expression in the maintenance and differentiation of neural stem cells (2012)
  • Author(s): Kageyama, R
  • Organizer: The 4th International Congress on Stem Cells and Tissue Formation.
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2012-07-20
• [Presentation] Ultradian rhythms in somite segmentation and other biological events (2012)
  • Author(s): Kageyama R
  • Organizer: BSDB/BSCB/JSDB Joint Spring Meeting
  • Place of Presentation: Warwick,UK
  • Year and Date: 2012-04-18
• [Remarks]
  • URL: http://www.kyoto-u.acjp/ja/news_data/h/hlinews6/2012/121207_1. htm
• [Remarks]
  • URL: http://www.kyoto-u.acjp/en/news_data/h/h1/news6/2012/121207_1. htm
• [Remarks]
  • URL: http://www.virus.kyoto-u.acjp/about/y2012/kageyama20121226.html