Development of newt molecular genetic systems

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657155
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Tottori University
• Principal Investigator: TAKEUCHI Takashi 鳥取大学, 医学部, 教授 (70197268)
• Co-Investigator(Renkei-kenkyūsha): HAYASHI Toshinori 鳥取大学, 医学部, 准教授 (60580925)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 発生 / 再生 / 有尾両生類 / 分子遺伝学 / トランスジェニック / イベリアトゲイモリ / 近交系 / 純系

Research Abstract

An experimental model system for molecular genetics in newts is necessary to clarify the mechanisms of regeneration from the perspective of gene functions. We have introduced Iberian ribbed newt (Pleurodeles waltl) as the suitable model newts. In the results of our researches, we have been able to shorten the periods for sexual maturation, and are developing 2 inbred and various transgenic lines of P. waltl newts. We also demonstrated that Cre-loxP recombination system efficiently worked in the newts. In addition, we achieved targeted gene knockout in the newt using TALENs. These results show that conditional knockout newts would be available by combination of Cre-loxP recombination and TALENs. Our system would be very powerful for studying the molecular mechanisms of not only regeneration but also various researches. In relation to the result of this research, we published total 12 research and review papers, also our group had 29 conference presentations.

Research Products

• [Journal Article] Repression of cyclin D1 expression is necessary for the maintenance of cell cycle exit in adult mammalian cardiomyocytes (2014)
  • Author(s): Tane, S., Kubota, M., Okayama, H.,Ikenishi, A., Yoshitome, S., Iwamoto,N., Satoh, Y., Kusakabe, A., Ogawa, S.,Kanai, A., Molkentin, J. D., Nakamura, K., Ohbayashi, T., and Takeuchi, T.
  • Journal Title: J. Biol. Chem Volume: (in press)
  • DOI: 10.1074/jbc.M113.541953
  • Peer Reviewed
• [Journal Article] Regulation of cardiomyocyte proliferation during development and regeneration (2014)
  • Author(s): Takeuchi, T.
  • Journal Title: Dev. Growth. Differ Volume: (in press)
  • DOI: 10.1111/dgd.12134
  • Peer Reviewed
• [Journal Article] CDK inhibitors, p21 ^< Cip1> and p27 ^< Kip1>, participate in cell cycle exit of mammalian cardiomyocytes (2014)
  • Author(s): Tane, S., Ikenishi, A., Okayama, H.,Iwamoto, N., Nakayama, I. K., and Takeuchi, T.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 443 Pages: 1105-1109
  • DOI: 10.1016/j.bbrc.2013.12.109
  • Peer Reviewed
• [Journal Article] Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration (2014)
  • Author(s): Hayashi, T., Sakamoto, K., Sakuma,T., Yokotani, N., Inoue, T., Kawaguchi,E., Agata, K., Yamamoto, T., and Takeuchi, T.
  • Journal Title: Dev. Growth Differ Volume: 56 Pages: 115-121
  • DOI: 10.1111/dgd.12103
  • Peer Reviewed
• [Journal Article] 再生研究の革命戦士～イベリアトゲイモリの奮闘～ (2014)
  • Author(s): 林 利憲、竹内 隆
  • Journal Title: 細胞工学 Volume: 33 Pages: 203-207
• [Journal Article] 種間による自律的な心臓再生能力の違いは何によって決まるのか? (2014)
  • Author(s): 竹内 隆、林 利憲
  • Journal Title: 実験医学 Volume: 32 Pages: 29-35
• [Journal Article] Histone H3 Lysine 9 methyltransferases, G9a and GLP are essential for cardiac morphogenesis.Mech (2013)
  • Author(s): Inagawa, M., Nakajima, K., Makino,T., Ogawa, S., Kojima, M., Ito, S.,Ikenishi, A., Hayashi, T., Schwartz, R.J., Nakamura, K., Obayashi, T.,Tachibana, M., Shinkai, Y., Maeda, K.,Miyagawa-Tomita, S., and Takeuchi,T.
  • Journal Title: Dev Volume: 130 Pages: 519-531
  • DOI: 10.1016/j.mod.2013.07.002
  • Peer Reviewed
• [Journal Article] Molecular genetic system for regenerative studies using newts (2013)
  • Author(s): Hayashi, T., Yokotani, N., Tane, S.,Matsumoto, A., Myouga, A., Okamoto,M., and Takeuchi, T.
  • Journal Title: Dev. Growth Differ Volume: 55 Pages: 229-236
  • DOI: 10.1111/dgd.12019
  • Peer Reviewed
• [Journal Article] 発生過程から成体にかけての心筋細胞の増殖パターンはどのように作られ、どのような意義があるのか? (2013)
  • Author(s): 竹内 隆、池西 愛子、岡山 仁美、岩本 典子
  • Journal Title: 実験医学 Volume: 31 Pages: 205-211
• [Journal Article] Cell cycle regulation in mouse heart duringembryonic and postnatal stages (2012)
  • Author(s): Ikenishi, A., Okayama, H., Iwamoto,N., Yoshitome, S., Tane, S., Nakamura, K., Obayashi, T., Hayashi, T., and Takeuchi, T.
  • Journal Title: Dev.Growth Differ Volume: 54 Pages: 731-738
  • DOI: 10.1111/j.1440-169X.2012.01373.x
  • Peer Reviewed
• [Journal Article] DNA damage signaling triggers degradation of histone methyltransferases through APC/CCdh1 in senescent cells (2012)
  • Author(s): Takahashi, A., Imai, Y., Yamakoshi,K., Kuninaka, S., Ohtani, N.,Yoshimoto, S., Hori, S., Tachibana, M.,Anderton, E., Takeuchi, T., Shinkai, Y., Peters, G., Saya, H., and Hara, E.
  • Journal Title: Mol. Cell Volume: 45 Pages: 123-131
  • DOI: 10.1016/j.molcel.2011.10.018
  • Peer Reviewed
• [Journal Article] Notch prosensory effects in the Mammalian cochlea are partially mediated by Fgf20 (2012)
  • Author(s): Munnamalai, V., Hayashi, T.,Bermingham-McDonogh, O.
  • Journal Title: J. Neurosci Volume: 12 Pages: 12876-12884
  • DOI: 10.1523/JNEUROSCI.2250-12.2012
  • Peer Reviewed
• [Presentation] Molecularmechanisms of cell proliferation in cardiomyocytes during cardiac development and regeneration (2014)
  • Author(s): Takeuchi, T., Tane, S., Okayama,Ikenishi, A., H., Matsumoto, A.,Myouga, A., Hayashi, T.
  • Organizer: CDB Symposium 2014
  • Place of Presentation: RIKEN CDB 神戸市
  • Year and Date: 20140310-12
  • Invited
• [Presentation] Molecular genetic system for regenerative studies using newts Iberian Ribbed Newts (Pleurodeles waltl) (2014)
  • Author(s): Hayashi, T., Yokotani, N., Matsumoto,A., Myouga, A., Sakamoto, K., Inoue,T., Kawaguchi, E., Agata, K., Sakuma,T., Yamamoto, T., Takeuchi, T.
  • Organizer: CDB Symposium 2014
  • Place of Presentation: RIKEN CDB、神戸市
  • Year and Date: 20140310-12
• [Presentation] Linage tracing analysis of cardiomyocytes in the newt cardiac regeneration by tamoxifen-inducible Cre-loxP system (2014)
  • Author(s): Myouga, A., Yokotani, N., Takeuchi, T.,Hayashi, T.
  • Organizer: CDB Symposium 2014
  • Place of Presentation: RIKENCDB、神戸市
  • Year and Date: 20140310-12
• [Presentation] イモリの心臓再生における心筋細胞の増殖と、再生心筋組織に対する寄与 (2014)
  • Author(s): 林 利憲、茗荷あゆみ、横谷 直樹、竹内隆
  • Organizer: 日本再生医療学会
  • Place of Presentation: 国立京都国際会館 京都市
  • Year and Date: 20140304-06
• [Presentation] 心筋細胞の増殖制御と心臓再生 (2014)
  • Author(s): 竹内隆
  • Organizer: シンポジウム 幹細胞の増殖・分化と再生創薬
  • Place of Presentation: 米子コンベンションセンター米子市
  • Year and Date: 2014-02-21
  • Invited
• [Presentation] イモリの心臓再生過程における細胞系譜の追跡と再生心筋に対する寄与の解明 (2013)
  • Author(s): 茗荷 あゆみ,横谷 直樹,竹内 隆,林利憲
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場神戸市
  • Year and Date: 20131203-06
• [Presentation] マウス心筋細胞のM 期阻害機構はいつ獲得されるのか? (2013)
  • Author(s): 岡山 仁美,田根 将志,池西 愛子,竹内 隆
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場 神戸市
  • Year and Date: 20131203-06
• [Presentation] 哺乳類心筋細胞のM 期進行阻害機構は心傷害によって解除されるか? (2013)
  • Author(s): 池西 愛子,田根 将志,岡山 仁美,竹内 隆
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場 神戸市
  • Year and Date: 20131203-06
• [Presentation] TALEN によるイモリのゲノム編集の可能性と、再生研究への展開 (2013)
  • Author(s): 林 利憲、坂本興亮、佐久間哲史、横谷直樹、井上武、川口恵里、阿形清和、山本卓、 竹内隆
  • Organizer: ゲノム編集研究会
  • Place of Presentation: 広島大学、東広島市
  • Year and Date: 20131026-27
• [Presentation] 遺伝子操作、ゲノム編集技術を用いたイモリ再生研究の展開 (2013)
  • Author(s): 林 利憲、茗荷 あゆみ、 横谷 直樹、佐久間 哲史、 山本 卓、 竹内 隆
  • Organizer: 日本動物学会招待講演
  • Place of Presentation: 岡山大学 岡山市
  • Year and Date: 20130926-28
• [Presentation] 両生類のイモリを用いた心臓再生機構の解明 (2013)
  • Author(s): 林利憲、横谷直樹、松本晃、茗荷あゆみ、川口恵理、井上武、阿形清和、竹内隆
  • Organizer: 日本再生医療学会
  • Place of Presentation: パシフィコ横浜 横浜市
  • Year and Date: 20130821-23
• [Presentation] Molecularmechanisms of cell proliferation in cardiomyocytes during cardiacdevelopment and regeneration (2013)
  • Author(s): Takeuchi, T., Tane, S., Okayama,Ikenishi, A., Yokotani, N., Matsumoto,A., Myouga, A., Hayashi, T.
  • Organizer: 日本発生生物学会
  • Place of Presentation: くにびきメッセ 松江市
  • Year and Date: 20130528-31
  • Invited
• [Presentation] Linage tracing analysis ofcardiomyocytes in the newt cardiac regeneration by tamoxifen-inducible Cre-loxP system (2013)
  • Author(s): Myouga, A., Yokotani, N., Takeuchi, T.,Hayashi, T.
  • Organizer: 日本発生生物学会
  • Place of Presentation: くにびきメッセ 松江市
  • Year and Date: 20130528-31
• [Presentation] What molecule triggersre-proliferation of cardiomyocytes during newt heart regeneration? (2013)
  • Author(s): Yokotani, N., Inoue, T., Kawaguchi, E.,Agata, K., Takeuchi, T., Hayashi, T.
  • Organizer: 日本発生生物学会
  • Place of Presentation: くにびきメッセ 松江市
  • Year and Date: 20130528-31
• [Presentation] Molecular genetic systemfor regenerative studies using newt (2013)
  • Author(s): Hayashi, T., Yokotani, N., Matsumoto,A., Myouga, A., Okamoto, M.,Takeuchi, T.
  • Organizer: 日本発生生物学会
  • Place of Presentation: くにびきメッセ 松江市
  • Year and Date: 20130528-31
• [Presentation] なぜ、哺乳類は心臓を再生できず、イモリはできるのか? (2013)
  • Author(s): 竹内隆、田根将志、横谷直樹、岡山仁美、池西愛子、松本晃、茗荷あゆみ、林利憲
  • Organizer: 日本再生医療学会
  • Place of Presentation: パシフィコ横浜 横浜市
  • Year and Date: 20130321-23
  • Invited
• [Presentation] 心筋細胞の増殖制御と心臓再生 (2013)
  • Author(s): 竹内 隆
  • Organizer: 西日本心臓血管発生研究会
  • Place of Presentation: 広島大学、広島市
  • Year and Date: 2013-10-26
  • Invited
• [Presentation] Molecular mechanisms of cell cycle arrest in cardiomyocytes during cardiac development and regeneration (2012)
  • Author(s): Takeuchi, T., Tane, S., Ikenishi, A.,Okayama, H., Yokotani, N.,Matsumoto, A., Hayashi, T.
  • Organizer: 日本分子生物学会 指定演者
  • Place of Presentation: 福岡国際会議場 福岡市
  • Year and Date: 20121211-14
• [Presentation] How doadult mammalian cardiomyocytesmaintain the proliferation arrest? (2012)
  • Author(s): Tane, S., Yoshitome, Satoh, Y.,Iwamoto, N., Okayama, H., Ikenishi,A., Hayashi, T., Takeuchi, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場 福岡市
  • Year and Date: 20121211-14
• [Presentation] How does cell cycle ofmouse cardiomyocytes arrest? : analysis of cell cycle regulator (2012)
  • Author(s): Ikenishi, Iwamoto, N., Tane, S.,Okayama, H., Satoh, Y., Hayashi, T.,Takeuchi, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場 福岡市
  • Year and Date: 20121211-14
• [Presentation] How does cell cycle of mouse cardiomyocytes arrest? : analysis of cell cycle pattern (2012)
  • Author(s): Okayama, H., Tane, S., Iwamoto, N.,Ikenishi, A., Satoh, Y., Hayashi, T.,Takeuchi, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場 福岡市
  • Year and Date: 20121211-14
• [Presentation] Molecular geneticsystem for regenerative studiesusing newt (2012)
  • Author(s): Hayashi, T., Yokotani, N., Tane, S.,Matsumoto, A., Myouga, A.,Takeuchi, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場福岡市
  • Year and Date: 20121211-14
• [Presentation] What molecule triggers reproliferationof cardiomyocytesduring newt heart regeneration? (2012)
  • Author(s): Yokotani, N., Matsumoto, A.,Hayashi, T., Inoue, T., Shibata, N.,Kawaguchi, E., Agata, K., Takeuchi,T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場 福岡市
  • Year and Date: 20121211-14
• [Presentation] 心筋細胞の増殖制御機構と心臓再生 : マウスとイモリとを比較する (2012)
  • Author(s): 竹内隆、田根将志、岡山仁美、池西愛子、岩本典子、横谷直樹、松本晃、林利憲
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪大学 豊中市
  • Year and Date: 20120913-15
  • Invited
• [Presentation] イモリ心臓再生における心筋細胞増殖の時空間パターンの解明 (2012)
  • Author(s): 林利憲、松本晃、横谷直樹、川口恵理、西村 理、井上武、阿形清和、竹内隆
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪大学豊中市
  • Year and Date: 20120913-15
  • Invited
• [Presentation] Howdo adult mammalian cardiomyocytesmaintain the proliferation arrest? (2012)
  • Author(s): Tane, S., Yoshitome, Satoh, Y.,Iwamoto, N., Okayama, H., Ikenishi,A., Hayashi, T., Takeuchi, T.
  • Organizer: 日本発生生物学会、細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場 神戸市
  • Year and Date: 20120528-31
• [Presentation] Development of the model system formolecular genetics for theregene-rative studies in urodeles (2012)
  • Author(s): Hayashi, T., Yokotani, N., Matsumoto,A., Tane, S., Takeuchi, T.
  • Organizer: 日本発生生物学会、細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場 神戸市
  • Year and Date: 20120528-31
• [Presentation] Analysis ofthe expression patterns of Cell cycleregulator genes during newt cardiac egeneration (2012)
  • Author(s): Matsumoto, A., Hayashi, T., Yokotani,N., Kawaguchi, E., Nishimura, O.,Agata, K., Takeuchi, T.
  • Organizer: 日本発生生物学会、細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場 神戸市
  • Year and Date: 20120528-31
• [Presentation] 心筋細胞の増殖制御機構と心臓再生 : なぜ、イモリは心臓再生が可能で哺乳類は不可能なのか? (2012)
  • Author(s): 竹内隆
  • Organizer: 分子病理学研究会
  • Place of Presentation: 恵那峡グランドホテル 恵那市
  • Year and Date: 2012-07-22
• [Book] 両生類におけるTALEN を用いた遺伝子改変 実験医学別册 今すぐ始めるゲノム編集 (2014)
  • Author(s): 林 利憲、坂根 祐人、竹内 隆、鈴木 賢一(山本卓編集)
  • Total Pages: 180-188
  • Publisher: 羊土社
• [Remarks]
  • URL: http://toridaimedseitai.web.fc2.com/index.html
• [Remarks]
  • URL: http://www.med.tottori-u.ac.jp/biosign/5982.html