Utilization of regeneration-inducing genes to transform recalcitrant species.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658007
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: Kagawa University
• Principal Investigator: KYO Masaharu 香川大学, 農学部, 教授 (70195395)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 個体再生 / 形質転換

Outline of Final Research Achievements

Using a chemical-induced gene expression system we examined the effect of several transcription factor genes related to morphogenesis or organogenesis in Arabidopsis on the regeneration in in vitro culture of explants of some recalcitrant crops and Nicotiana species. The cDNAs were amplified by RT-PCR using total RNA prepared from seedling or inflorescence of Arabidopsis, integrated into pER8 harboring XVE expression system and transferred to recalcitrant crops and Nicotiana through Agroinfection method. In the recalcitrant species induction of transformants or transgenic organs were not successful so far. However, using hybrid lines of transgenic tobacco, we found that the co-expression of a pair of transgenes caused the regeneration at much higher frequency than single transgene expression did alone. This finding suggests the possibility to develop new transformation-vectors for inducing in vitro regeneration from explants of different species.

Free Research Field

植物細胞生理学