Establishment of universal site-directed mutagenesis system via gene targeting

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658011
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: SAIKA Hiroaki 独立行政法人農業生物資源研究所, 農業生物先端ゲノム研究センター ゲノム機能改変研究ユニット, 主任研究員 (20435613)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ジーンターゲッティング / 変異導入 / マーカー除去

Outline of Final Research Achievements

Gene targeting (GT) can introduce desirable mutations into a targeted gene as expected. In this study, we aimed to advance site-directed mutagenesis technology in which GT and subsequent marker elimination via DNA repair system, single strand annealing (SSA). We succeeded in the enrichment of marker-free cells by means of conferring a herbicide tolerance to cells in which SSA events occurred.

Free Research Field

植物分子育種学