Development of a systematic method to screen DNAs to make gene expression stable and analysis of the DNAs.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658012
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: National Agriculture and Food Research Organization (2014); National Institute of Agrobiological Sciences (2012-2013)
• Principal Investigator: KISHIMOTO Naoki 独立行政法人農業・食品産業技術総合研究機構, 作物研究所企画管理室, 企画チーム長 (00370651)
• Co-Investigator(Renkei-kenkyūsha): MITSUHARA Ichiro 独立行政法人農業生物資源研究所, 植物科学研究領域, 上級研究員 (80370683)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオテクノロジー / 植物 / 遺伝子 / サイレンシング / 育種学

Outline of Final Research Achievements

To screen novel DNA fragments showing ability to circumvent position effects in eukaryote genomes, including transcriptional gene silencing (TGS) of transgenes, I planned to establish a method for screening DNAs suppress TGS (Anti-silencing regions: ASRs) from plant genome and to try screening of random DNA library from plant genome using the method. To develop a systematic method to screen such DNAs, I applied the concept of the screening method in Kishimoto et al. (2013), which use a silenced transgenic tobacco plant in which secondary introduced transgene shows obligatory promoter-homology dependent TGS, to this study. I developed a screening method to assay abilities of DNAs suppressing TGS, based on the phenomena that Arabidopsis FWA genomic transgene shows obligatory TGS in the genome. Using this method, I determined which region to suppress TGS most efficiently in ASR602 found by Kishimoto et al. (2013). However I could not construct random genomic library for the screening.

Free Research Field

高等植物の分子遺伝学