The molecular mechanism of devernalization in relation to FLC gene expression in Japanese radish

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658026
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kyoto University
• Principal Investigator: DOI Motoaki 京都大学, (連合)農学研究科(研究院), 教授 (40164090)
• Co-Investigator(Kenkyū-buntansha): HOSOKAWA Munetaka 京都大学, 大学院農学研究科, 准教授 (40301246)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 園芸学 / ダイコン / 春化 / 脱春化 / 分子機構 / RsFLC

Outline of Final Research Achievements

Japanese radish (Raphanus sativus L.) is a seed-vernalization-type plant requiring chilling for flowering．It is also known that exposure of seeds to high temperature has a devernalization effect. The purpose of this study is to clarify the molecular aspects of vernalization and devernalization of Japanese radish by investigating the expressions of some flowering-related genes. In 'Hayabutori-syogoinn', the expression level of RsFLC decresesed with increased duration of chilling and thereafter the expression levels of RsFT and RsSOC1 increased prior to flowering. Exposure of vernalized seeds with 9 days' chilling to high temperature caused devernalization and the RsFLC level was restored a little. Close relations between vernalized or devernalized status and the expression level of RsFLC were observed in 'Hayabutori-syogoinn' and 'Wakayama', but in 'Tokinashi' the low expression levels of RsFLC after exposure to 18 days' chilling did not result in flowering.

Free Research Field

園芸科学