Basic study for determination of proteins relating on trafficking of mRNA

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658027
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kyoto University
• Principal Investigator: HOSOKAWA Munetaka 京都大学, (連合)農学研究科(研究院), 准教授 (40301246)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: mRNA / キクわい化ウイロイド / 移動性

Outline of Final Research Achievements

In this experiment, we determined the distribution of chrysanthemum stunt viroid in chrysanthemum cells and from this result it was revealed that CSVd is suitable as model RNA molecules for screening of proteins which helps trafficking of RNA. And transgenic plants with over-expression of partial CSVd sequences were generated. These plants can be used for the determination of important sequences for traffic CSVd RNA to nucleus and this information is valuable for screening of trafficking proteins with CSVd. And we compared RNA expression in CSVd-infected and -non infected tobacco plants and could screened 8 genes of candidates for trafficking proteins. Now these genes are under being analyzed in detail. In addition, we developed the protein-screening method using chrysanthemum plants, which are strong resistant to CSVd. The transgenic plants over expressed of CSVd in these resistant cultivars were prepared here.

Free Research Field

蔬菜花卉園芸学