2014 Fiscal Year Final Research Report
Activity based metaproteomic analysis of nitrous oxide reductase in soil
Project/Area Number |
24658063
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plant nutrition/Soil science
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Research Institution | Yamaguchi University |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | 一酸化二窒素還元酵素 / 二次元電気泳動 / 活性染色 / 土壌 / 脱窒菌 / 一酸化二窒素 |
Outline of Final Research Achievements |
This study attempted to extract active nitrous oxide reductases (N2ORs) of denitrifying bacteria in soil and to analyze their diversity by detecting them based on the activity-dependent staing on a two-dimensional native PAGE gel. It was not successfule to avoid isoelectric precipitation of the proteins during the isoelectric electrophoresis, the first step of the 2-D native PAGE. The activity of N2OR preparations was lost inreversively. The extraction of active N2ORs from soil was conducted by indirect methods where bacterials cells were separated from soil particles by density gradient centrifugation. The amount of proteins thus extracted was not sufficient to create a Coomacy Brilliant Blue-positive spots on the native PAGE gel.
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Free Research Field |
土壌微生物学
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