Mechanism of resistance to osmotic stress by translation inhibitors

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658066
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Hirosaki University
• Principal Investigator: HYOUTA Himeno 弘前大学, 農学生命科学部, 教授 (80208785)
• Co-Investigator(Renkei-kenkyūsha): MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 翻訳阻害剤 / 塩ストレス / 浸透圧ストレス / リボソーム / 翻訳 / シグマE / ppGpp

Research Abstract

Effects of various kinds of drugs on Escherichia coli cell were examined and we found inhibitors of protein synthesis such as chloramphenicol, kasgamycin or tetracycline increase resistance to osmotic (salt) stress. By using qRT-PCR, we also found that these protein synthesis inhibitors prematurely and transiently activate these membrane-related sigma factor, sigma E.
Resistance to osmotic (salt) stress is also provided by serine hydroxamate, which produces ppGpp. We finally found that there are two pathways for salt resistance, ppGpp-dependent and ppGpp-independent pathways.

Research Products

• [Journal Article] Mechanism of trans-translation revealed by invitrostudies. (2014)
  • Author(s): Himeno, H., Kurita, D., Muto, A.
  • Journal Title: Frontiersin Microbiology Volume: Vol.5 Pages: 65
  • DOI: 10.3389/fmicb.2014.00065
  • Peer Reviewed
• [Journal Article] Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence. (2014)
  • Author(s): Yang, Z., Guo, Q., Goto, S., Chen, Y., Li, N., Yan, K., Zhang, Y., Muto, A., Deng, H., Himeno, H., Lei, J., Gao, N.
  • Journal Title: Protein & Cell Volume: Vol.5 Pages: 394-407
  • DOI: 10.3389/fmicb.2014.00065
• [Journal Article] tmRNA-mediated trans-translation as the major ribosome rescue system in abacterial cell. (2014)
  • Author(s): Himeno, H., Kurita, D., Muto, A.
  • Journal Title: Frontiers in Genetics Volume: Vol.5 Pages: 66
  • DOI: 10.3389/fgene.2014.00066
  • Peer Reviewed
• [Journal Article] Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process. (2013)
  • Author(s): Guo, Q., Goto, S., Chen, Y., Feng, B., Xu, Y., Muto, A., Himeno, H., Deng, H., Lei, J., Gao, N.
  • Journal Title: Nucleic Acids Res. Volume: Vol.41 Pages: 2609-2620
  • DOI: 10.1093/nar/gks1256
  • Peer Reviewed
• [Journal Article] GTPases involved in bacterial ribosome maturation. (2013)
  • Author(s): Goto, S., Muto, A., Himeno, H.
  • Journal Title: J. Biochem. Volume: Vol.153 Pages: 403-414
  • DOI: 10.1093/jb/mvt022
  • Peer Reviewed
• [Journal Article] 2つの機能を有する tmRNAによる細菌の翻訳解消システム (2013)
  • Author(s): 姫野俵太、栗田大輔、武藤昱
  • Journal Title: 実験医学 Volume: Vol.31,No.7 Pages: 54-60
• [Journal Article] Impairment of ribosome maturation or function confers salt resistance on Escherichia coli cells. (2013)
  • Author(s): Hase, Y., Tarusawa, T., Muto, A., Himeno, H.
  • Journal Title: PLoS ONE Volume: Vol.8 Pages: e65747
  • DOI: 10.1371/journal.pone.0065747
  • Peer Reviewed
• [Journal Article] In vitro trans-translation assay. (2012)
  • Author(s): Kurita, D., Muto, A., Himeno, H.
  • Journal Title: Methods in Molecular Biology Volume: Vol.905 Pages: 311-325
  • DOI: 10.1007/978-1-61779-949-5_20
  • Peer Reviewed
• [Presentation] tmRNA:non-coding RNAと coding RNAのハイブリッド (2013)
  • Author(s): 姫野俵太、栗田大輔、武藤昱
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20131202-06
• [Presentation] RsgA(リボソーム小サブユニット依存GTP加水分解酵素)の機能およびリボソームとの相互作用 (2013)
  • Author(s): 後藤史門、長谷要一、菊地岳志、栗田大輔、武藤昱、竹本千重、横山茂之、Sean R.Connell、Paola Fucini、姫野俵太
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 20130724-26
• [Presentation] Recognition of mRNA length on the ribosome by tmRNA andSmpB. (2013)
  • Author(s): Kurita, D., Miller, M.R., Muto, A., Buskirk, A.R., Himeno, H.
  • Organizer: Ribosomes Conference 2013
  • Place of Presentation: Napa Valley, California
  • Year and Date: 20130709-12
• [Presentation] GTPaseRsgAの機能と大腸菌リボソーム生合成の過程 (2013)
  • Author(s): 後藤史門、武藤あきら、姫野俵太
  • Organizer: 第10回21世紀大腸菌研究会
  • Place of Presentation: 修善寺
  • Year and Date: 20130620-21
• [Presentation] リボソームを介した浸透圧耐性機構 (2013)
  • Author(s): 樽澤武房、長谷要一、後藤史門、武藤あきら、姫野俵太
  • Organizer: 第2回リボソームミーティング
  • Place of Presentation: 東京
  • Year and Date: 20130328-29
• [Presentation] tmRNA/SmpBによる停滞したリボソームの認識機構 (2013)
  • Author(s): 栗田大輔、Mickey Miller、武藤あきら、Allen Buskirk、姫野俵太
  • Organizer: 第2回リボソームミーティング
  • Place of Presentation: 東京
  • Year and Date: 20130328-29
• [Presentation] Interplay of a GTPase and a ribosome-binding factor on the 30S subunit during a late stage of the bacterial ribosome biosynthesis. (2012)
  • Author(s): Goto, S., Muto, A., Himeno, H.
  • Organizer: The 9th International Conference on RibosomeSynthesis
  • Place of Presentation: Banff
  • Year and Date: 20120822-26
• [Presentation] The first translocation in trans-translation. (2012)
  • Author(s): Takada, K., Kurita, D., Naoe, C., Yokogawa, T., Himeno, H., Takemoto, C.
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 仙台
  • Year and Date: 20120718-20
• [Presentation] 線虫 SL1トランス.スプライシングは生殖細胞でより活発? (2012)
  • Author(s): 加藤新、佐藤洋旭、高橋大輝、姫野俵太、武藤あきら、牛田千里
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 仙台
  • Year and Date: 20120718-20
• [Book] 弘前大学知の散歩道 (2013)
  • Author(s): 姫野俵太
  • Total Pages: 9
  • Publisher: 弘前大学出版会
• [Book] 生命分子を統合する RNAーその秘められた役割と制御機構 (2013)
  • Author(s): 姫野俵太、栗田大輔、武藤昱
  • Total Pages: 7
  • Publisher: 羊土社
• [Remarks]
  • URL: http://hirosaki-rna.org/himeno/