2013 Fiscal Year Final Research Report
Establishment of intestinal peptide delivery system based on lactic acid bacteria and the therapeutic application for inflammatory bowel disease
| Project/Area Number |
24658099
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
MIAKE Fumio 福岡大学, 薬学部, 教授 (50248522)
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| Co-Investigator(Kenkyū-buntansha) |
KASHIGE Nobuhiro 福岡大学, 薬学部, 教授 (80185751)
SATHO Tomomitsu 福岡大学, 薬学部, 助教 (90369025)
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| Research Collaborator |
HIRAMATSU Yukihiro 福岡大学, 大学院・薬学研究科, 大学院生
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 乳酸菌 / CTB-YVAD融合体 / 抗炎症作用 / 炎症性腸疾患 |
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| Research Abstract |
Recombinant lactic acid bacteria (LAB) are used as bacterial vectors to deliver functional peptides to the intestine, and cholera toxin B subunit (CTB) can translocate into intestinal epithelial cells through GM1 ganglioside. To deliver the YVAD peptide, which has potential as a therapeutic agent for inflammatory bowel disease (IBD), to the intestine, we constructed LAB secreting a recombinant fusion protein of CTB with YVAD (CTB-YVAD). The present study demonstrated that CTB-YVAD bind to GM1 ganglioside, translocate into human intestinal epithelial cell line, and show the anti-inflammatory effects. Taken together, we showed potencies of peptide delivery system to the intestine used LAB and CTB, and new therapeutic agent for IBD used LAB secreting CTB-YVAD.
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[Journal Article] Expression and secretion of cholera toxin B subunit in lactobacilli2013
Author(s)
Takahiro Okuno, Nobuhiro Kashige, Tomomitsu Satho, Keiichi Irie, Yukihiro Hiramatsu, Tanjina Sharmin, Yuki Fukmits, Saori Uyeda, Seitaro Yamada, Tetsuya Harakuni, Takeshi Miyata, Takeshi Arakawa, Masumi Imoto, Akihisa Toda, Yukihiko Nakashima, Fumio Miake
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Journal Title
Biological Pharmaceutical Bulletin
Volume: 36(6)
Pages: 952-958
URL
Peer Reviewed
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[Journal Article] Differences in TLR9-dependent inhibitory effects of H_2O_2-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species2013
Author(s)
Yukihiro Hiramatsu, Tomomitsu Satho, Keiichi Irie, Shota Shiimura, Takahiro Okuno, Tanjina Sharmin, Yuki Fukumitsu, Saori Uyeda, Yukihiko Nakashima, Fumio Miake, Nobuhiro Kashige
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Journal Title
Microbes and Infection
Volume: 15(2)
Pages: 96-104
DOI
Peer Reviewed
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[Journal Article] Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus casei inhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells
Author(s)
Yukihiro Hiramatsu, Masatatsu Yamamoto, Tomomitsu Satho, Keiichi Irie, Akiko Kai, Saori Uyeda, Yuki Fukumitsu, Akihisa Toda, Takeshi Miyata, Fumio Miake, Takeshi Arakawa, Nobuhiro Kashige
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Journal Title
BMC Biotechnology
Volume: (In press)
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