UDP-Glc independent transglucosidase to mediate jasmonic acid degradation in rice

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658102
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Hokkaido University
• Principal Investigator: MATSUURA Hideyuki 北海道大学, (連合)農学研究科(研究院), 准教授 (20344492)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Jasmonic acid / 植物ホルモン

Outline of Final Research Achievements

In order to uncover controling system of jasmonate signaling pathway, we attempted to find out UDP-glucose independent transglucosylation enzyme, and OsBGlu1 was picked up to afford 12-glucopyranosyl jasmonic acid and 12-glucopuranosyljasmonoyl L-isoleucine. The biological activities of jasmonic acid, 12-hydroxyjasmonoyl L-isoleucine and 12-glucopyranosyljasmonoyl L-isoleucine were comparied, which revealed that jasmonic acid has most effective activity and 12-hydroxyjasmonoyl L-isoleucine has the lowest activity. Thus, our project showed that transglucosylation reaction mediated by OsBGlu1 is important pathway to control the activity of jasmonic acid.

Free Research Field

天然物有機化学