2013 Fiscal Year Final Research Report
The mechanism by which oocytes/embryos are injured at low/high temperatures
| Project/Area Number |
24658238
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Magosaburo 高知大学, 教育研究部総合科学系, 教授 (60152617)
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 低温傷害 / ブタ卵子 / TRPチャンネル |
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| Research Abstract |
We examined whether temperature-sensitive transient receptor potential (TRP) channels were involved in the injury of pig oocytes/embryos at low/high temperatures. Pig immature oocytes and maturing oocytes expressed mRNA of TRPA1, a cold-sensitive TRP channel, whereas mature oocytes and embryos expressed not only mRNA of TRPA1 but also that of TRPM8, another cold-sensitive TRP channel. TRPV1, a hot-sensitive channel, was expressed only in immature oocytes. The viability of pig oocytes decreased after cold treatment, depending on the activation temperature of the TRP channels expressed in oocytes at each stage. When oocytes were suppressed with the expression of the cold-sensitive TRP channels, the viability was improved markedly. These results strongly suggest that cold-sensitive TRP channels are involved in the chilling injury of pig oocytes.
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[Journal Article] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes : its relevance to their higher tolerance to cryopreservation2013
Author(s)
Bo Jin, Ryu-ichi Higashiyama, Yu-ichi Nakata, Jun-ichi Yonezawa, Shangdan Xu, Masashi Miyake, Sei-ichi Takahashi, Kazuhiro Kikuch, Ken-ichi Yazawa, Shuhei Mizobuchi, Saori Niimi, Mizuho Kitayama, Chihiro Koshimoto, Kazutsugu Matsukawa, Magosaburo Kasai, Keisuke Edashige
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Journal Title
Biology of Reproduction
Volume: 89
Pages: 1-12
DOI
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[Presentation] The role of aquaporin 9 in the movement of DMSO and acetamide in mouse morulae2012
Author(s)
Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai
Organizer
SSR's 45th Annual Meeting of Society of the Study of Reprocution
Place of Presentation
The Alumni Hall at the Hetzel Union Building, The Pennsylvania State University, University Park, Pennsylvania.
Year and Date
20120812-15
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