2014 Fiscal Year Final Research Report
Epigenetic analysis of the germ cell-specific genes in chicken germ-line specific cells
Project/Area Number |
24658246
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Basic veterinary science/Basic zootechnical science
|
Research Institution | Kyushu University |
Principal Investigator |
HATTORI Masa-aki 九州大学, (連合)農学研究科(研究院), 教授 (60175536)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | エピジェネティック / ニワトリ生殖細胞 / 精子 / 生殖細胞マーカー遺伝子 / 未分化細胞マーカー遺伝子 / メチル化遺伝子 |
Outline of Final Research Achievements |
Germ cell-specific genes such as Ddx4, Dnd1, and Dazl play critical roles in the proliferation and survival of germ cells. The methylation levels of these genes and the pluripotency marker gene in chicken sperm were investigated in relation to gene expression. The putative promoters of these genes showed very low methylation levels in sperm, but they were highly methylated in the liver. Conversely, the Alb gene promoter was highly methylated in sperm. The results that low methylation was in germ cell-specific genes but high methylation in non-specific genes may indicate selective DNA methylation of specific genes in chicken sperm. In addition, these germ cell-specific genes and the pluripotency marker gene may be ready to express any time after fertilization.
|
Free Research Field |
生殖内分泌学、時間生物学、発生生物学
|