Studies on novel gene expression regulation system by anti-sense RNA in herpesvirus multiplication

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658256
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Gifu University
• Principal Investigator: FUKUSHI Hideto 岐阜大学, 応用生物科学部, 教授 (10156763)
• Co-Investigator(Kenkyū-buntansha): OHYA Kenji 岐阜大学, 応用生物科学部, 准教授 (50402219)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: アンチセンス鎖 / ヘルペスウイルス / 遺伝子発現制御

Research Abstract

Genetic information is transcribed from DNA to RNA as mRNA. And the RNA called anti-sense RNA is transcribed from the antisense strand of DNA. The purpose of this study was to clarify the function of the anti-sense RNA found in equine herpesvirus infected cells. The miRNA was investigated and found that expression of viral miRNA was almost nothing. On the other hand, miRNA derived from the host genome showed variation of expression level according to the infection of virus. Transcription initiation sites were identified including the anti-sense RNAs. Double stranded RNA was extracted from the infected cells but their nucleotide sequences were not determined in the present study.