2013 Fiscal Year Final Research Report
Development of an automated microscopic system for optogenetics using a mercury lamp fluorescence microscope
| Project/Area Number |
24659087
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
EGAMI Youhei 香川大学, 医学部, 助教 (80432780)
KAWAI Katsuhisa 香川大学, 医学部, 助教 (80534510)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAKE Katsuya 香川大学, 医学部, 准教授 (30219745)
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| Research Collaborator |
KATO Takuma 香川大学, 医学部, 助教 (70625673)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 顕微鏡技術 / 細胞・組織 / 光制御 / シグナル伝達 / 分子スイッチ / 蛍光顕微鏡 / オプトジェネティクス |
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| Research Abstract |
Photomanipulation of genetically encoded photoactivatable proteins, also known as optogenetics, is one of the most innovative techniques in the fields of cell biology. Although photomanipulation is usually performed by diverting the photobleaching mode of a confocal laser microscope, photobleaching by the laser scanning unit is not always suitable for photoactivation. Therefore, we attempted to develop a simple automated wide-field fluorescence microscopy system for optogenetics. An automated fluorescence microscope can be controlled through MetaMorph imaging software, making it possible to acquire time-lapse, multiwavelength images of live cells. We wrote a macro program to change the excitation filter for photoactivation and illumination area during the intervals of image acquisition. When this program was run on the microscope, cells expressing photoactivatable Rac1 showed lamellipodial extension and cell surface ruffling in the illuminated region.
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[Journal Article] Development of an automated fluorescence microscopy system for photomanipulation of genetically encoded photoactivatable proteins (optogenetics) in live cells2014
Author(s)
Araki, N., Ikeda, Y., Kawai, K., Egami, Y., Miyake, K., Tsurumaki, N., Yamaguchi, M.
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Peer Reviewed
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