2013 Fiscal Year Final Research Report
Establishiment of calcium influx and detection system in the cilia
| Project/Area Number |
24659089
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YOKOYAMA Takahiko 京都府立医科大学, 医学(系)研究科(研究院), 教授 (70191525)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIBA Dai 独立行政法人・宇宙航空研究開発機構, 有人宇宙ミッション本部宇宙環境利用センター, 主任研究員 (50360722)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 繊毛 / カルシウム |
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| Research Abstract |
To establish a system that can get an influx of calcium and detect calcium in the cilia, we made channel-rhodopsin and cameleon(YC3.6) constructs linked to several cilia localization signals.These signals include Arl13B, Fiblocystin C-terminal and nphp3 N terminal. YC3.6 linked NPHP3 N-terminal and Arl13B with palmitoylation site successfully located to the cilia.Channel-rhodopsin linked to Arl13B also located to the cilia without palmitoylation site, suggesting that membrane protein such as channel-rhodopsin does not require palmitoylation to be localized to the cilia.We gave photo-stimulation to cells introduced channel-rodopsin. Unfortunately, calcium was not detected in these cells. To get effective calcium influx, an amplifier system may be required.
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Research Products
(8 results)
