2013 Fiscal Year Final Research Report
Development of the epithelium-like complex membrane system
| Project/Area Number |
24659096
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 脂質平面膜 / 液滴界面2重膜法 / 溶液急速灌流 |
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| Research Abstract |
To develop the epithelium-like complex membrane system, we start to establish a single-membrane system with a reproducible way. Compared to the conventional droplet interface bilayer method, the size of the droplet was decreased to one tenth, and the background noise was substantially attenuated. The asymmetric membrane was formed by adding different liposomes composed of distinct lipid compositions to the droplets. We revealed that the gating of the KcsA potassium channel was regulated by the acidic lipid in the inner leaflet of the membrane. The rapid perfusion system was introduced into the bilayer system, in which two glass pipettes were inserted into one of the droplet. The solution was exchanged within several tens of ms, and the pH-dependent activation of the KcsA channel was readily measured.
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[Journal Article] Biased Brownian Stepping Rotation of F_oF_1-ATP Synthase Driven by Proton Motive Force2013
Author(s)
Watanabe, R., Tabata, K.V., Iino, R., Ueno, H., Iwamoto, M., Oiki, S. and Noji, H.
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Journal Title
Nature Commun
Volume: 4
Pages: 1631
DOI
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[Journal Article] ATR-FTIR Spectroscopy Revealed the Different Vibrational Modes of the Selectivity Filter Interacting with K^+ and Na^+ in the Open and Collapsed Conformations of the KcsA Potassium Channel2012
Author(s)
Furutani, Y., Shimizu, H., Asai, Y., Fukuda, T., Oiki, S. and Kandori, H.
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Journal Title
J. Phys. Chem. Lett.
Volume: 3
Pages: 3806-3810
DOI
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[Journal Article] Functional Equilibrium of the KcsA Structure Revealed by NMR2012
Author(s)
Imai, S., Osawa, M., Mita, K., Toyonaga, S., Machiyama, A., Ueda, T., Takeuchi, K., Oiki, S. and Shimada, I.
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Journal Title
J . Biol. Chem.
Volume: 287
Pages: 39634-39641
DOI
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[Remarks] 老木成稔:イオンチャネルの動的構造と分子機構解明のための1分子研究.科研費NEWS2013年度Vol.2, 17, 2013
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[Remarks] 日本生理学会 サイエンストピックスNo. 82岩本真幸(老木成稔):K^+チャネルが細胞膜の脂質の環境を感じ取り活性を制御する機構の解明
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[Remarks] 日本生理学会 サイエンストピックスNo. 92角野歩(老木成稔):カリウムチャネルKcsAのゲート開閉と連動した膜中集合・離散ダイナミクス
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