2013 Fiscal Year Final Research Report
Identification of plasma membrane leucine receptor and elucidation of its link to mTOR signaling pathway
| Project/Area Number |
24659116
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
KANAI Yoshikatsu 大阪大学, 医学(系)研究科(研究院), 教授 (60204533)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAMORI Shushi 大阪大学, 医学系研究科, 准教授 (90467572)
OHGAKI Ryuichi 大阪大学, 医学系研究科, 助教 (20467525)
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| Co-Investigator(Renkei-kenkyūsha) |
HARI Yoshiyuki 大阪大学, 薬学研究科, 特任准教授 (50347423)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 受容体 / チャネル / 輸送系 / シグナル情報伝達系 / 比較定量プロテオミクス / リン酸化プロテオミクス |
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| Research Abstract |
By comparative quantitative proteomics using mass spectrometry, several candidates for the plasma membrane leucine receptor were identified. Functional analysis of these candidates revealed that one protein plays a role in mTORC1 pathway downstream of growth factor receptors and thereby regulates the activity of mTORC1 cooperatively with amino acids. We also developed a new analytical procedure of mass spectrometry suitable for integral membrane proteins by improving the sample preparation process and mass spectrometric measurement, because the detection and identification of integral membrane proteins were difficult in general. Additionally, to reveal the overall signaling events downstream of leucine receptor, we established a method for quantitative phosphoproteomics. The technical basis fundamentally important for the identification of leucine receptor has been established in this study.
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Research Products
(21 results)
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[Journal Article] Down-regulation of placental mTOR, insulin/IGF-I signaling, and nutrient transporters in response to maternal nutrient restriction in the baboon2014
Author(s)
Kavitha JV, Rosario FJ, Nijland MJ, McDonald TJ, Wu G, Kanai Y, Powell TL, Nathanielsz PW, Jansson T
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Journal Title
FASEB J.
Volume: Vol.28
Pages: 1294-1305
DOI
Peer Reviewed
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[Journal Article] Clinical significance of L-type amino acid transporter 1 expression as a prognostic marker and potential of new targeting therapy in biliary tract cancer2013
Author(s)
Kaira K, Sunose Y, Ohshima Y, Ishioka NS, Arakawa K, Ogawa T, Sunaga N, Shimizu K, Tominaga H, Oriuchi N, Itoh H, Nagamori S, Kanai Y, Yamaguchi A, Segawa A, Ide M, Mori M, Oyama T, Takeyoshi I
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Journal Title
BMC Cancer.
Volume: Vol.13
Pages: 482
DOI
Peer Reviewed
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[Journal Article] NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane2012
Author(s)
Hagiwara K, Nagamori S, Umemura YM, Ohgaki R, Tanaka H, Murata D, Nakagomi S, Nomura KH, Kage-Nakadai E, Mitani S, Nomura K, Kanai Y
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Journal Title
PLoS One.
Volume: Vol.7
Pages: e43050
DOI
Peer Reviewed
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[Journal Article] Prognostic significance of L-type amino-acid transporter 1 expression in surgically resected pancreatic cancer2012
Author(s)
Kaira K, Sunose Y, Arakawa K, Ogawa T, Sunaga N, Shimizu K, Tominaga H, Oriuchi N, Itoh H, Nagamori S, Kanai Y, Segawa A, Furuya M, Mori M, Oyama T, Takeyoshi I
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Journal Title
Br J Cancer.
Volume: Vol.107
Pages: 632-638
DOI
Peer Reviewed
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[Journal Article] Transport of 3-fluoro-L-α-methyl-tyrosine by tumor-upregulated L-type amino acid transporter 1 : a cause of the tumor uptake in PET2012
Author(s)
Wiriyasermkul P, Nagamori S, Tominaga H, Oriuchi N, Kaira K, Nakao H, Kitashoji T, Ohgaki R, Tanaka H, Endou H, Endo K, Sakurai H, Kanai Y
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Journal Title
J Nucl Med.
Volume: Vol.53
Pages: 1253-1261
DOI
Peer Reviewed
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