Identification of transcription regulatory factor using chromatinized DNA template immobilized on microbeads

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659121
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI Hidehisa 北海道大学, 医学(系)研究科(研究院), 助教 (30423544)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 転写因子 / 転写制御因子 / HNF4 alpha / ES細胞

Research Abstract

Many evidence including that all of the Yamanaka factors necessary for generation of IPS cells are transcription factors indicates that transcription factors play an very important role in regulation of gene expression. To address the mechanism how transcription factor regulates gene transcription, we performed the identification of transcription regulatory factor using chromatinized DNA template.

Research Products

• [Journal Article] The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway (2014)
  • Author(s): Sato T., Takahashi H., Hatakeyama S., Iguchi A. and Ariga T.
  • Journal Title: Oncogene Volume: 31;0
  • Peer Reviewed
• [Journal Article] Identification of anti-Sez6l2 antibody in a patient with cerebellar ataxia and retinopathy (2014)
  • Author(s): Yaguchi H, Yabe I, Takahashi H, Okumura F, Takeuchi A, Horiuchi K, Kano T, Kanda A, Saito W, Matsumoto M, Nakayama KI, Hatakeyama S, Sasaki H
  • Journal Title: Journal of Neurology Volume: 261(1) Pages: 224-226
  • DOI: 10.1007/s00415-013-7134-5
  • Peer Reviewed
• [Journal Article] Transcriptional Elongation Factor Elongin A Regulates Retinoic Acid-Induced Gene Expression during Neuronal Differentiation of Embryonic Stem Cells (2012)
  • Author(s): Yasukawa T, Bhatt S, Takeuchi T, Kawauchi J, Takahashi H, Tsutsui A, Muraoka T, Inoue M, Tsuda M, Kitajima S, Conaway RC, Conaway JW, Trainor PA, Aso T
  • Journal Title: Cell reports Volume: 29;2(5) Pages: 1129-1136
  • DOI: 10.1016/j.celrep.2012.09.031
  • Peer Reviewed
• [Journal Article] TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis (2012)
  • Author(s): Yaguchi H, Okumura F, Takahashi H, Kano T, Kameda H, Uchigashima M, Tanaka S, Watanabe M, Sasaki H, Hatakeyama S
  • Journal Title: J Biol Chem Volume: 287(15) Pages: 12050-12059
  • DOI: 10.1074/jbc.M111.307678
  • Peer Reviewed
• [Journal Article] Role for the human mediator subunit Med26 in transcription elongation (2012)
  • Author(s): Takahashi H
  • Journal Title: Seikagaku Volume: 84(7) Pages: 577-581
  • Peer Reviewed
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2013)
  • Author(s): 高橋秀尚,瀧川一学, Delnur Anwar,柴田美音,佐藤チエリ,佐藤滋生, Ranjan Amol, Chris W. Seidel,築山忠維,渡部昌,林正康,大川恭行, Joan W. Conaway, Ronald C. Conaway,畠山鎮次
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸ポートピアホテル(神戸)
  • Year and Date: 20131203-05
• [Presentation] Human Mediator subunit Med26 regulates the transcription of small nuclear RNA genes through the recruitment of little elongation complex (2013)
  • Author(s): Hidehisa Takahashi, Chieri Tomomori-Sato, Shigeo Sato, Amol Ranjan, Masayasu Hayashi, Yasuyuki Ohkawa, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: Mechanism of eukaryotic transcription, Cold spring harbor meeting
  • Year and Date: 20130827-30
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚, Joan W. Conaway, Ronald C. Conaway,畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡(福岡)
  • Year and Date: 20121214-16
• [Presentation] TRIM29によるTRRAP/Tip60複合体の機能制御とがん化との関わり (2012)
  • Author(s): 桝田安志,高橋秀尚,畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡(福岡)
  • Year and Date: 20121214-16
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚,佐藤智信, Conaway, J.W., Conaway, R.C.,畠山鎮次
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡(福岡)
  • Year and Date: 20121211-14
• [Remarks]
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/