Basic Research on Tissue Regeneration for Radiation Emergency Medicine

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659140
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kawasaki Medical School
• Principal Investigator: INUBUSHI Masayuki 川崎医科大学, 医学部, 准教授 (70399830)
• Co-Investigator(Kenkyū-buntansha): MICHIKAWA Yuichi 独立行政法人放射線医学総合研究所, 緊急被ばく医療研究センター, 主任研究員 (20360688)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 再生医学 / 分子イメージング

Research Abstract

We extracted stem cells from various organs of a transgenic mouse systemically and constitutively expressing the sodium/ iodide symporter (NIS) gene (NIS-Tg mouse), which was originally developed by this principle investigator. We found adipose-derived stem cells showed higher 99mTc uptake than bone marrow stem cells in NIS-Tg mice. We transplanted 100000 adipose-derived stem cells of NIS-Tg mice into mice after irradiation to one side of the body, and 48 hours later we succeeded in detecting apparent signals at the transplanted region with 99mTc SPECT imaging. The transplanted cells could be tracked for more than 1 month in the same living mice, and we discussed the significance from the viewpoint of regenerative medicine.

Research Products

• [Journal Article] FDG uptake heterogeneity evaluated by fractal analysis improves the differential diagnosis of pulmonary nodules (2014)
  • Author(s): Miwa K, Inubushi M, Wagatsuma K, Nagao M, Murata T, Koyama M, Koizumi M, Sasaki M
  • Journal Title: Eur J Radiol Volume: 83(4) Pages: 715-719
  • DOI: 10.1016/j.ejrad.2013.12.020
  • Peer Reviewed
• [Journal Article] Drugs interacting with organic anion transporter-1 affect uptake of Tc-99m-mercaptoacetyl-triglycine (MAG3) in the human kidney : therapeutic drug interaction in Tc-99m-MAG3 diagnosis of renal function and possible application of Tc-99m-MAG3 for drug development (2013)
  • Author(s): Takahara N, Saga T, Inubushi M, Kusuhara H, Seki C, Ito S, Oyama N, Yokoyama O, Sugiyama Y, Fujibayashi Y
  • Journal Title: Nucl Med Biol Volume: 40(5) Pages: 643-650
  • DOI: 10.1016/j.nucmedbio.2013.03.006
  • Peer Reviewed
• [Journal Article] Predictive value of 3'-deoxy-3'-[18F]fluorothymidine positron emission tomography/computed tomography for outcome of carbon ion radiotherapy in patients with head and neck mucosal malignant melanoma (2013)
  • Author(s): Inubushi M, Saga T, Koizumi M, Takagi R, Hasegawa A, Koto M, Wakatuki M, Morikawa T, Yoshikawa K, Tanimoto K, Fukumura T, Yamada S, Kamada T
  • Journal Title: Ann Nucl Med Volume: 27(1) Pages: 1-10
  • DOI: 10.1007/s12149-012-0652-x
  • Peer Reviewed
• [Journal Article] Single-photon emission computed tomography of spontaneous liver metastasis from orthotopically implanted human colon cancer cell line stably expressing human sodium/iodide symporter reporter gene (2012)
  • Author(s): Inubushi M, Jin YN, Murai C, Hata H, Kitagawa Y, Saga T
  • Journal Title: EJNMMI Res Volume: 2(1) Pages: 46
  • DOI: 10.1186/2191-219X-2-46
  • Peer Reviewed
• [Journal Article] Novel human monoclonal antibody against epidermal growth factor receptor as an imaging probe for hepatocellular carcinoma (2012)
  • Author(s): Sogawa C, Tsuji AB, Yoshida C, Inubushi M, Furukawa T, Koizumi M, Akahori Y, Ukai Y, Kurosawa G, Kurosawa Y, Saga T
  • Journal Title: Nucl Med Commun Volume: 33(7) Pages: 719-725
  • DOI: 10.1097/MNM.0b013e3283531d68
  • Peer Reviewed
• [Journal Article] Long-term effects of hepatocyte growth factor gene therapy in rat myocardial infarct model (2012)
  • Author(s): Jin YN, Inubushi M, Masamoto K, Odaka K, Aoki I, Tsuji AB, Sagara M, Koizumi M, Saga T
  • Journal Title: Gene Ther Volume: 19(8) Pages: 836-843
  • DOI: 10.1038/gt.2011.128
  • Peer Reviewed
• [Presentation] マウス間葉系幹細胞の皮下移植による放射線誘発白毛化の抑制 (2014)
  • Author(s): 道川祐市、福崎智子、後藤希、徐華、犬伏正幸、松崎有美、田嶋克史
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館,京都
  • Year and Date: 20140304-06
• [Presentation] Comparison of Intratumoral 18FDG and 18FAZA Distribution in Non-small Cell Lung Cancer Using Deformable Image Registration (2013)
  • Author(s): Sasaki M, Koizumi M, Murata T, Wagatsuma K, Saga T, Inubushi M, Tachibana H, Miwa K
  • Organizer: The 99th Scientific Assembly and Annual Meeting of the Radiological Society of North America
  • Place of Presentation: McCormick Place, Chicago, IL
  • Year and Date: 20131201-06
• [Presentation] FDG Uptake Heterogeneity Evaluated by Fractal Analysis Improves the Differential Diagnosis of Pulmonary Nodules (2013)
  • Author(s): Sasaki M, Koizumi M, Umeda T, Okawa A, Umeda T, Wagatsuma K, Inubushi M, Miwa K
  • Organizer: The 99th Scientific Assembly and Annual Meeting of the Radiological Society of North America
  • Place of Presentation: McCormick Place, Chicago, IL
  • Year and Date: 20131201-06
• [Presentation] Noninvasive spatial and temporal tracking of multilayered cell sheet on infarcted heart using reporter gene imaging (2013)
  • Author(s): Otani K, Zeniya T, Kawashima H, Fukuda H, Hashikawa Y, Moriguchi T, Inubushi M, Iida H, Yamahara K
  • Organizer: American Heart Association Scientific Sessions 2013
  • Place of Presentation: Dallas Convention Center, Dallas, TX
  • Year and Date: 20131116-20
• [Presentation] The imaging of tumor hypoxia with 18F-FMISO and 18F-FAZA in comparison to the imaging of hypoxic response with radioiodine using sodium/iodine symporter (NIS) reporter gene in human xenograft tumors (2013)
  • Author(s): Takeuchi Y, Inubushi M, Xu H, Hata H, Kitagawa Y, Saga T
  • Organizer: Society of Nuclear Medicine and Molecular Imaging 2013 Annual Meeting
  • Place of Presentation: Vancouver Convention Centre, Vancouver, Canada
  • Year and Date: 20130608-12
• [Presentation] Comparison of intratumoral distribution of 18FDG and 18FAZA using deformable image registration procedure in non-small cell lung cancer patients (2013)
  • Author(s): Miwa K, Tachibana H, Inubushi M, Wagatsuma K, Murata T, Takiguchi T, Koizumi M, Saga T, Sasaki M
  • Organizer: The 69th Annual Scientific Congress of the Japanese Society of Radiological Technology
  • Place of Presentation: Pacifico Yokohama, Yokohama
  • Year and Date: 20130411-14
• [Presentation] Predictive value of 3'-deoxy-3'-[18F]fluorothymidine positron emission tomography/computed tomography for outcome of carbon ion radiotherapy in patients with head and neck mucosal malignant melanoma (2012)
  • Author(s): Inubushi M, Saga T, Koizumi M, Takagi R, Hasegawa A, Koto M, Wakatuki M, Morikawa T, Yoshikawa K, Tanimoto K, Fukumura T, Yamada S, Kamada T
  • Organizer: Annual Congress of the European Association of Nuclear Medicine 2012
  • Place of Presentation: Milano Congressi (MiCo), Milan, Italy
  • Year and Date: 20121027-31
• [Presentation] マウス自然発生肝転移モデルのSPECTイメージング (2012)
  • Author(s): 犬伏正幸、竹内康人、村井知佳、秦浩信、北川善政、佐賀恒夫
  • Organizer: 第52回日本核医学会学術総会
  • Place of Presentation: さっぽろ芸術文化の館/ロイトン札幌(札幌)
  • Year and Date: 20121011-13
• [Presentation] Tracking of transplanted stem cells in myocardial infarction model rat : validation of simultaneous dual-isotope SPECT imaging with Tc-99m and Tl-201 (2012)
  • Author(s): Zeniya T, Kawashima H, Otani K, Fukuda H, Hashikawa Y, Koshino K, Hori Y, Moriguchi T, Enmi J, Iguchi S, Yamamoto A, Miyake Y, Inubushi M, Iida H
  • Organizer: 2012 World Molecular Imaging Congress
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 20120905-08
• [Presentation] Clinical differentiation between malignant and benign pulmonary nodules using heterogeneity of 18F-FDG uptake distribution (2012)
  • Author(s): Miwa K, Wagatsuma K, Sasaki M, Nagao M, Koyama M, Inubushi M, Koizumi M
  • Organizer: 2012 Society of Nuclear Medicine Annual Meeting
  • Place of Presentation: Miami Beach, FL
  • Year and Date: 20120609-13
• [Presentation] Molecular genetic imaging of hypoxia response using sodium/iodide symporter (NIS) reporter gene (2012)
  • Author(s): Takeuchi Y, Inubushi M, Xu H, Mio K, Hata H, Kitagawa Y, Saga T
  • Organizer: 2012 Society of Nuclear Medicine Annual Meeting
  • Place of Presentation: Miami Beach, FL
  • Year and Date: 20120609-13
• [Book] 核医学技術総論改訂版 (2014)
  • Author(s): 犬伏正幸, 他
  • Publisher: 山代印刷(京都)(印刷中)
• [Remarks]
  • URL: http://kwweb-res.kawasaki-m.ac.jp/kwmhp/KgApp?kyoinId=Kgyysggk