2013 Fiscal Year Final Research Report
Identification of genes that regulate allergic diseases.
| Project/Area Number |
24659151
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OKAZAKI Taku 徳島大学, 疾患プロテオゲノム研究センター, 教授 (00362468)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | アレルギー / 免疫学 / ゲノム / モデル動物 / 免疫補助受容体 |
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| Research Abstract |
To analyze the role of immuno-inhibitory receptors in the regulation of allergic diseases, we have backcrossed PD-1 and LAG-3 deficient mice on NC mice that spontaneously develop atopic dermatitis under conventional condition. PD-1 deficiency did not induce atopic dermatitis under SPF condition suggesting that PD-1 deficiency cannot overcome the requirement of pathogenic microbes for the development of atopic dermatitis in NC mice. On the other hand, some NC-LAG-3KO mice developed severe atopic dermatitis even under SPF condition. Unfortunately, the colony of NC-LAG-3KO mice was quite unstable by unknown reason and we failed to backcross LAG-3KO mice on NC mice more than 6 generations. Therefore, we could not have obtain a decisive data regarding the role of LAG-3 in the development of atopic dermatitis in NC mice so far. We are planing to use anti-LAG-3 blocking Abs or other allergic models to analyze the role of LAG-3 in the regulation of allergic responses.
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