Live imaging of experimental cerebral malaria

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659189
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: YUI Katsuyuki 長崎大学, 医歯(薬)学総合研究科, 教授 (90274638)
• Co-Investigator(Renkei-kenkyūsha): TAMURA Takahiko 長崎大学, 大学院医歯薬学総合研究科, 助教 (00434035)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: マラリア / イメージング / 感染 / 脳

Research Abstract

I planned to investigate the cellular mechanisms underlying the pathogenesis of experimental cerebral malaria using 3 imaging techniques; luciferase imaging, fluorescence imaging using multiphoton microscope, and PET/CT imaging. I was able to get enough information and preparation for the analysis using these techniques.

Research Products

• [Journal Article] Flt3 ligand treatment modulates rodent parasitemia infection via MyD88 and IFN-g-dependent mechanisms (2014)
  • Author(s): Tamura, T, Akbari, M., Kimura K., Kimura, D., Yui, K.
  • Journal Title: Parasite Immunol Volume: 36 Pages: 87-99
• [Journal Article] CD8^+ T cells specific for a malaria cytoplasmic antigen form clusters around infected hepatocytes and are protective at the liver stage of infection (2013)
  • Author(s): Kimura, K., Kimura, D., Matsushima, Y., Miyakoda, M., Honma, K., Yuda, M., Yui, K.
  • Journal Title: Inf. Immun Volume: 81(10) Pages: 3825-3834
• [Journal Article] CD19(+) B cells confer protection against experimental cerebral malaria in semi-immune rodent model (2013)
  • Author(s): Bao, L.Q., Huy, N.T., Kikuchi, M., Yanagi, T., Senba, M., Shuaibu, M.N., Honma, K., Yui, K., Hirayama, K.
  • Journal Title: PLos One Volume: 8(5) Pages: e64836
• [Journal Article] Closeup : Cross-presentation of malaria antigen by brain microvessels : why CD8^+ T cells are critical for the pathogeneisis of cerebral malaria (2013)
  • Author(s): Yui,K.
  • Journal Title: EMBO Mol. Med Volume: 5(7) Pages: 899-901
• [Journal Article] Accumulation of MHC class II^+ CD11c^- non-lymphoid cells in the spleen during infection with Plasmodium yoelii is lymphocyte-dependent (2013)
  • Author(s): Kamei, R., Miyakoda, M., Tamura, T., Kimura, D., Honma, K., Kimura, K., Yui, K.
  • Journal Title: Microbiol. Immunol Volume: 57(3) Pages: 213-223
• [Journal Article] Development of memory CD8^+ T cells and their recall responses during blood-stage infection with Plasmodium berghei ANKA (2012)
  • Author(s): Miyakoda, M., Kimura, D. , Honma, K., Kimura, K., Yuda, M., Yui, K.
  • Journal Title: J. Immunol Volume: 189(9) Pages: 4396-4404
• [Journal Article] The species specificity of immunity generated by live whole organism immunization with erythrocytic and pre-erythrocytic stages of rodent malaria parasites and implications for vaccine development (2012)
  • Author(s): Inoue M., Jianxia T., Miyakoda M., Kaneko O., Yui, K., Culleton R.
  • Journal Title: Int. J. Parasitol Volume: 42 Pages: 859-870
• [Journal Article] Platelets presents antigen in the context of MHC class I (2012)
  • Author(s): Chapman, L.M., Aggrey, A.A., Field, D.J., Srivastava, K., Ture S., Yui, K., Topham, D.J., Baldwin III, W,M., Morell, C.N.
  • Journal Title: J. Immunol Volume: 189(2) Pages: 916-923
• [Presentation] マラリア原虫特異的CD4^+ T細胞は、IL-27を介してCD4^+ T細胞の防御機能を抑制する (2014)
  • Author(s): 木村大輔、都田真奈、木村一美,本間季里、原博満、堀昌平、吉田裕樹、由井克之
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 松山市
  • Year and Date: 20140327-28
• [Presentation] 赤血球期マウスマラリア原虫感染における従来型樹状細胞の減少はI型、II型インターフェロン依存的細胞死である (2014)
  • Author(s): 田村隆彦、木村一美、由井克之、吉田栄人
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 松山市
  • Year and Date: 20140327-28
• [Presentation] T cell responses during infection with Plasmodium berghei ANKA (2014)
  • Author(s): K. Yui
  • Organizer: Mini symposium on malaria immunopathology, IFReC
  • Place of Presentation: 吹田市
  • Year and Date: 2014-01-15
• [Presentation] IL-27 is produced by CD4^+T cells during infection with malaria parasites and inhibits protective immune responses (2013)
  • Author(s): KIMURA D, MIYAKODA M, KIMURA K, HONMA K, HARA H, HORI S, YOSHIDA H, YUI K
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉市
  • Year and Date: 20131211-13
• [Presentation] CD8 ^+T cells form clusters around infected hepatocytes protecting against liver-stage infextion with plasmodium berghei ANKA (2013)
  • Author(s): KIMURA K, KIMURA D, MIYAKODA M, YUI K
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉市
  • Year and Date: 20131211-13
• [Presentation] Flt3 ligand treatment inhibits parasitemia during infection with rodent malaria parasites via MyD88-and IFN-g-dependent mechanisms (2013)
  • Author(s): TAMURA T, KIMURA K, YUI K
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉市
  • Year and Date: 20131211-13
• [Presentation] CD8 + T細胞による肝細胞期マラリア原虫感染防御の生体イメージング (2013)
  • Author(s): 由井克之、木村一美、都田真奈、木村大輔、油田正夫
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京都
  • Year and Date: 20130329-31
• [Presentation] マラリア原虫特異的CD4T細胞はEBI3陽性サイトカインを介して他のCD4T細胞のIL-2産生を制する (2013)
  • Author(s): 木村大輔、都田真奈、本間季里、木村一美、原博満、吉田裕樹、由井克之
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京都
  • Year and Date: 20130329-31
• [Presentation] マラリア原虫感染における抗原特異的記憶CD8^+T細胞の二次応答抑制 (2013)
  • Author(s): 都田真奈、木村大輔、本間季里、木村一美、油田正夫、由井克之
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京都
  • Year and Date: 20130329-31
• [Presentation] Development of memory CD8 + T cells and their recall responses during blood-stage infection with Plasmodium berghei ANKA (2012)
  • Author(s): M. Miyakoda, D. Kimura, K. Honma, K. Kimura, M. Yuda, K. Yui.
  • Organizer: Immunological mechanisms of vaccination, Part of the Keystone sumposia global health series
  • Place of Presentation: オタワ(カナダ)
  • Year and Date: 20121213-18
• [Presentation] Regulation of T cell responses during infection with P. berghei ANKA leading to the protective immunity and pathogenesis of cerebral malaria (2012)
  • Author(s): D. Kimura, M. Miyakoda, K. Kimura, K. Yui
  • Organizer: The 6^<th> Nagasaki symposium on tropical and emerging infectious diseases, The 11 th Nagasaki-Singapore medical symposium
  • Place of Presentation: 長崎市
  • Year and Date: 20121210-12
• [Presentation] CD4^+ T cells produce EBI-3^+ cytokine inhibiting their own protective immune responses during infection with malaria parasites (2012)
  • Author(s): D. Kimura, M. Miyakoda, Honma, K. Kimura, H. Hara, H. Yoshida, K. Yui
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 20121205-07
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/mmi/im/