2013 Fiscal Year Final Research Report
Structure-function of bacterial mitoNEET homologs
Project/Area Number |
24659202
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Bacteriology (including Mycology)
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Research Institution | Nippon Medical School |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KUSANO Teruo 日本医科大学, 医学部, 助教 (30434129)
KUMASAKA Takashi 高輝度光科学研究センター, 利用研究促進部門, 副主席研究員 (30291066)
IWASAKI Hideo 早稲田大学, 理工学術院, 教授 (00324393)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Keywords | mitoNEET / 鉄硫黄クラスター / 金属蛋白質 / 構造機能生理 |
Research Abstract |
MitoNEET is a mammalian mitochondrial outer membrane iron-sulfur protein with a potential pharmacological and clinical target of pioglitazone, an insulin-sensitizer for the treatment of type II diabetes. In this study, we conducted the phenotypal analyses of a deletion strain and several mutant strains of a bacterial mitoNEET homolog (TthNEET) of Thermus thermophilus HB8 in comparison with the wild-type strain, and re-analyzed the available metabolome and microarray datasets of this thermophile. We also obtained the high-resolution X-ray diffraction datasets of mammalian mitoNEET in complex with several drug compounds.
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Research Products
(10 results)
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[Journal Article] Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin2012
Author(s)
Iwasaki, T., Fukazawa, R., Miyajima- Nakano, Y., Baldansuren, A., Matsushita, S., Lin, M. T., Gennis, R.B., Hasegawa, K., Kumasaka, T., Dikanov, S.A.
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Journal Title
J. Am. Chem. Soc
Volume: 134
Pages: 19731-19738
Peer Reviewed
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[Presentation] Expression host strains for selective isotope labeling of thermophile enzymes. (#AP.P 15)2013
Author(s)
Fukazawa, R., Lin, M. T., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Dikanov, S.A., Gennis, R.B., Iwasaki, T.
Organizer
Thermophiles 2013, 12th International Meeting at the University of Regensburg
Place of Presentation
Regensburg, Germany
Year and Date
20130908-13
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[Presentation] Thermophile homolog of mitoNEET from Thermus thermophilus HB8. (#FT.O 02)2013
Author(s)
Iwasaki, T., Hagiuda, E., Fukazawa, R., Hayashi-Iwasaki, Y., Oshima, T., Dikanov, S. A., Hasegawa, K., Kumasaka, T.
Organizer
Thermophiles 2013, 12th International Meeting at the University of Regensburg
Place of Presentation
Regensburg, Germany
Year and Date
20130908-13
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[Presentation] 2D pulsed EPR analysis of histidine ligand residue(s) of the thermophile Rieske and mitoNEET type iron-sulfur proteins. (Mz76)2013
Author(s)
Matsushita, S., Fukazawa, R., Iwasaki, T., Taguchi, A., Baldansuren, A., Dikanov, S.A.
Organizer
16th International Conference on Bioinorganic Chemistry
Place of Presentation
Grenoble, France
Year and Date
20130722-26
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[Presentation] Application of 2D pulsed EPR (HYSCORE) in the structural analysis of hydrogen bond network around a biological iron-sulfur cluster. (Mz54)2013
Author(s)
Iwasaki, T., Miyajima-Nakano, Y., Fukazawa, R., Hagiuda, E., Matsushita, S., Hasegawa, K., Kumasaka, T., Baldansuren, A., Dikanov, S.A.
Organizer
16th International Conference on Bioinorganic Chemistry
Place of Presentation
Grenoble, France
Year and Date
20130722-26
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[Presentation] Characterization of the extremophile homologs of mitoNEET. (#P117)2012
Author(s)
Hagiuda, E., Miyajima-Nakano, Y., Ohmori, D., Kusano, T., Matsushita, S., Dikanov, S.A., Kumasaka, T., Iwasaki, T.
Organizer
9th International Congress on Extremophiles
Place of Presentation
Sevilla, Spain
Year and Date
20120900
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[Presentation] Selective isotope labelling of extremophile metalloproteins. (#P119)2012
Author(s)
Fukazawa, R., Lin, M.T., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Choi, S. K., Dikanov, S.A., Gennis, R.B., Iwasaki, T.
Organizer
9th International Congress on Extremophiles
Place of Presentation
Sevilla, Spain
Year and Date
20120900
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