2014 Fiscal Year Final Research Report
New attempt of cold-chain free oral influenza vaccine development
| Project/Area Number |
24659203
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
FUKUYAMA Yoshiko
MEJIMA Mio
KUROKAWA Shiho
SUZUKI Yuji
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ワクチン |
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| Outline of Final Research Achievements |
By using a T-DNA vector driven by a prolamin promoter and an overexpression vaccine cassette with RNAi, we have established MucoRice-CTB-HA1 intended as a cold-chain free oral influenza vaccine. To investigate the immunogenicity of MucoRice-CTB-HA1, we subcutaneously or orally immunized mice. Although subcutaneous immunization induced CTB- and HA-specific IgG immune responses, oral immunization induced CTB-specific IgG and mucosal IgA immune responses but not HA-specific one. We now consider to improve the immunization system by using subcutaneous followed by oral immunization to induce systemic and mucosal HA-specific Ab immune responses.
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| Free Research Field |
粘膜免疫学
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