2013 Fiscal Year Final Research Report
Establishment of disease iPS cells and molecular analysis of age-related changes in progeroid syndrome
| Project/Area Number |
24659346
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Chiba University |
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Principal Investigator |
YOKOTE Koutaro 千葉大学, 医学(系)研究科(研究院), 教授 (20312944)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Takahiko 千葉大学, 大学院医学研究院, 寄附講座教員 (40301791)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Werner症候群 / iPS細胞 / 早老症候群 / 老化 / 遺伝子発現 |
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| Research Abstract |
Werner syndrome (WS) is a progeroid disorder caused by mutations in the WRN gene, which encodes a RecQ type DNA helicase. In order to clarify molecular mechanism of pathogenesis of WS, we gene-transferred Yamanaka's four factors, such as Oct-4, Sox2, Klf4, c-Myc, into skin fibroblasts of WS patients. We isolated iPS clone cells and confirmed them with highly proliferative ability as well as pluripotency, indicating establishment of WS iPS cells by reprogramming. Furthermore, we investigated the protective effect of read-thorough drugs on WS fibroblasts with a nonsense mutation and discussed the therapeutic potentiality of the drugs to WS.
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[Journal Article] Concurrent loss of Ezh2 and Tet2 cooperates in the pathogenesis of myelodysplastic disorders2013
Author(s)
Muto T, Sashida G, Oshima M, Wendt GR, Mochizuki-Kashio M, Nagata Y, Sanada M, Miyagi S, Saraya A, Kamio A, Nagae G, Nakaseko C, Yokote K, Shimoda K, Koseki H, Suzuki Y, Sugano S, Aburatani H, Ogawa S, Iwama A.
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Journal Title
J Exp Med
Volume: 210(12)
Pages: 2627-39
DOI
Peer Reviewed
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[Journal Article] Sitagliptin Improves Postprandial Hyperglycemia by Inhibiting Glucagon Secretion in Werner Syndrom With Diabetes2013
Author(s)
Watanabe K, Kobayashi K, Takemoto M, Ishibashi R, Yamaga M, Kawamura H, Fujimoto M, Ishikawa T, Ohnishi S, Okabe E, Peng He, Yokote K.
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Journal Title
Diabetes Care
Volume: 36
Pages: e119
DOI
Peer Reviewed
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[Journal Article] Executive summary of the Japan Atherosclerosis Society (JAS) guidelines for diagnosis and prevention of atherosclerotic cardiovascular diseases in Japan- 2012 version2013
Author(s)
Teramoto T, Sasaki J, Ishibashi S, Birou S, Daida H, Dohi S, Egusa G, Hiro T, Hirobe K, Iida M, Kihara S, Kinoshita M, Maruyama c, Ohta T, Okamura T, Yamashita S, Yokode M, Yokote K.
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Journal Title
J Atheroscler Thromb
Volume: 20 (6)
Pages: 517-523
DOI
Peer Reviewed
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[Presentation] Oxidative stress, aging and progeric syndrome. Recent progress in basic and clinical research on progeroid syndrome2013
Author(s)
Shimizu, T., Koyama, H., Shibuya, S., Shimamoto, A., Takemoto, M., Yokote, K.
Organizer
The 20^<th> IAGG World Congress of Gerontology and Geriatrics (IAGG 2013)
Place of Presentation
Seoul Korea
Year and Date
20130623-27
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[Presentation] Japanese diabetic patients with Werner syndrome exhibit high incidence of cancer2013
Author(s)
Onishi, S., Takemoto, M., Ishikawa, T., Okabe, E., Ishibashi, R., He, P., Kobayashi, K., Fujimoto, M., Kawamura, H., Yokote, K.
Organizer
20th World Congress of the International Association of Gerontology and Geriatrics (IAGG2013)
Place of Presentation
Seoul, Korea
Year and Date
20130623-27
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[Presentation] Incidence and characteristics of metabolic disorders and vascular complications in Werner syndrome patients in Japan2013
Author(s)
Okabe, E., Onishi, S., Takemoto, M., Ishikawa, T., Ishibashi, R., He, P., Kobayashi, K., Fujimoto, M., Kawamura, H., Yokote, K.
Organizer
20th World Congress of the International Association of Gerontology and Geriatrics (IAGG2013)
Place of Presentation
Seoul, Korea
Year and Date
20130623-27
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