2013 Fiscal Year Final Research Report
Identification of specific markers for cancer stem cells and anti-cancer therapy targeting cancer stem cells
| Project/Area Number |
24659363
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto University |
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Principal Investigator |
CHIBA Tsutomu 京都大学, 医学(系)研究科(研究院), 教授 (30188487)
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| Co-Investigator(Kenkyū-buntansha) |
SENO Hiroshi 京都大学, 医学研究科, 講師 (90335266)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 癌幹細胞 / 組織幹細胞 / Hes1 / Dckl1 / 大腸ポリープ / 癌幹細胞治療 |
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| Research Abstract |
We examined effects of targeting Hes1 and Dcamkl1, possible cancer stem cell markers, on colonic cancer cells. Hes1-specific siRNAs significantly reduced growth of human colonic cancer cells. In addition, we also examined the effect of Hes1-siRNA to ApcMin mice. Finally, we obtained Dckl1-positive cells from both normal intestine and polyps of ApcMin mice by FACS sorting, and found several transcripts specifically expressed in Dcamkl1-positive tumor cells. The siRNAs or inhibitors specific for the molecules exclusively expressed in Dcamkl1-positive tumor cells inhibited tumor cell growth without affecting normal epithelial cells. These data suggested possible anti-cancer strategy targeting cancer stem cells without affecting normal tissue stem cells.
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Research Products
(20 results)
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[Journal Article] Deletion of nardilysin prevents tieh development of steatohepatitis and liver fibrotic changes2014
Author(s)
Ishizu-Higashi S, Seno H, Nishi E, Matsumoto Y, Ikuta K, Tsuda M, Kimura Y, Takada Y, Kimura Y, Nakanishi Y, Kanda K, Komekado H, Chiba T.
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Journal Title
PLos One
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] A model of liver carcinogenesis originating from hepatic progenitor cells with accumulation of genetic alterations2014
Author(s)
Kim SK, Nasu A, Komori J, Shimizu T, Matsumoto Y, Minaki Y, Kohno K, Shimizu K, Uemoto S, Chiba T, Marusawa H.
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Journal Title
Int J Cancer
Volume: 134
Pages: 1067-1076
DOI
Peer Reviewed
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[Journal Article] Inhibitory role of Gas6 in intestinal tumorigenesis2013
Author(s)
Akitake-Kawano R, Seno H, Nakatsuji M, Kimura Y, Nakanishi Y, Yoshioka T, Kanda K, Kawada M, Kawada K, Sakai Y, Chiba T.
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Journal Title
Carcinogenesis
Volume: 34
Pages: 1567-1574
DOI
Peer Reviewed
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[Journal Article] Dclk1 distinguishes between tumor and normal stem cells in the intestine2013
Author(s)
Nakanishi Y, Seno H, Fukuoka A, Ueo T, Yamaga Y, Maruno T, Nakanishi N, Kanda K, Komekado H, Kawada M, Isomura A, Kawada K, Sakai Y, Yanagita M, Kageyama R, Kawaguchi Y, Taketo MM, Yonehara S, Chiba T.
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Journal Title
Nature Genet
Volume: 45
Pages: 98-103
DOI
Peer Reviewed
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[Journal Article] Nardilysin and ADAM proteases promote gastric cancer cell growth by activating intrinsic cytokine signaling via enhanced ectodomain shedding of TNFα2012
Author(s)
Kanda K, Komekado H, Sawabu T, Ishizu S, Nakanishi U, Nakatsuji M, Akitake-Kawano R, Ohono M, Hiraoka Y, Kawada M, Kawada K, Sakai Y, Matsumoto K, Kunichika M, Kimura T, Seno H, Nishi E, Chiba T.
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Journal Title
EMBO Mol Med
Volume: 4
Pages: 396-411
DOI
Peer Reviewed
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[Journal Article] Gastroesophageal reflux disease symptoms and dietary behaviors are significant correlates of short sleep duration in the general population : The Nagahama Study
Author(s)
Murase K, Tabara Y, Takahashi Y, Muro S, Yamada R, Setoh K, Kawaguchi M, Kadotani H, Kosugi S, Sekine A, Nakayama T, Mishima M, Chiba T, Chin K, Matsuda F.
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Journal Title
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Peer Reviewed
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