2013 Fiscal Year Final Research Report
Crosstalk between CagA and inflammation in H. pylori-related gastric carcinogenesis.
| Project/Area Number |
24659365
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka University |
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Principal Investigator |
TSUJII Masahiko 大阪大学, 医学(系)研究科(研究院), 准教授 (40303937)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEHARA Tetsuo 大阪大学, 大学院医学系研究科, 教授 (70335355)
IIJIMA Hideki 大阪大学, 大学院医学系研究科, 講師 (90444520)
NISHIDA Tsutomu 大阪大学, 大学院医学系研究科, 助教 (20467575)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 胃癌 / ヘリコバクターピロリ / CagA |
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| Research Abstract |
In in vitro experiments, CagA significantly attenuated let-7 expression leading to Ras pathway activation. CagA enhanced c-myc, DNA methyltransferase 3B (DNMT3B) and Enhancer of Zeste homologue 2 (EZH2) expression and attenuated miR-26a and miR-101 expression, which resulted in the attenuation of let-7 expression by histone and DNA methylation. Experiments performed in CagA transgenic mice revealed that c-myc, EZH2 and DNMT3B expression were enhanced and let-7 expression was attenuated to induce Ras oncoprotein expression in the stomach, with no associated inflammation. It is concluded that H pylori CagA induces aberrant epigenetic silencing of let-7 expression, leading to Ras upregulation.
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Research Products
(28 results)
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[Presentation] Cyclooxygenase-2の癌幹細胞バイオロジー、DNA異常メチル化に及ぼす影響.ワークショップ;消化管がん分子病態診断学の進歩2012
Author(s)
辻井正彦,近藤純平,赤坂智史,林義人,金瑛,加藤元彦,山本俊祐,井上拓也,山田拓哉,西田勉,渡部健二,飯島英樹,竹原徹郎
Organizer
第8回日本消化管学会
Place of Presentation
仙台
Year and Date
20120000
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[Presentation] ESD後の適切なフォローアップ方法～多発胃癌に対するサーベイランスの観点から～ESDフォーラム; Where is our destination?2012
Author(s)
加藤元彦,西田勉,山本克己,北村信次,由雄敏之,中村剛之,小森真人,河相直樹,西原彰浩,中西文彦,中原征則,荻山秀治,木下和郎,辻井正彦,竹原徹郎
Organizer
第8回日本消化管学会
Place of Presentation
仙台
Year and Date
20120000
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