2013 Fiscal Year Final Research Report

A challenge of lung regeneration by iPS cells

Research Project

Project/Area Number	24659396
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Respiratory organ internal medicine
Research Institution	Niigata University
Principal Investigator	SAIJO YASUO 新潟大学, 医歯学系, 教授 (10270828)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	iPS 細胞 / 肺再生 / 肺障害
Research Abstract	We transplanted alveolar differentiated iPS cells into the lung scaffolds. After 12 days culture in vitro, Differentiated iPS cells reconstructed the alveoli and over 10% cells were positive for SPC or Tia. In the bleomycin-induced lung injury model mouse, the cells were transplanted intratracheally. Bronchoalveolar lavage demonstrated IL-6 and TNF-a were drastically suppressed in the differentiated iPS cells group compared to the controls. Fibrotic changes were also suppressed in differentiated iPS cells group. SPC+/PKH26+ or T1a+/PKH26+ cells were observed in the differentiated iPS cells group, but positive cells were quite few. In this study, we demonstrated that alveolar differentiated iPS cells could reconstruct alveoli of the lung scaffold, and ameliorate lung fibrosis in the bleomycin-induced lung injury model mouse.