2012 Fiscal Year Final Research Report
Development of rat model of TDP-43 propagation
| Project/Area Number |
24659425
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KANOUCHI Tadashi 東京医科歯科大学, 医学部附属病院, 助教 (50345287)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOTA Takanori 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (90231688)
OHKUBO Takuya 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (90587461)
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| Project Period (FY) |
2012
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| Keywords | ALS / ラット / TDP-43 / 伝播 |
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| Research Abstract |
We developed rat model of TDP-43 propagation by injecting AAV1-IRES-hrGFP vector expressing flag-tagged human wild-type TDP-43 to the anterior horn of the 6th spinal cord of Fischer rat. After 2 weeks of injection, exogenous TDP-43 was expressed much more extensively than hrGFP in the spinal cord. This rat model might show three patterns of propagation mechanism of exogenous TDP-43, which are locally cell-to-cell propagation, radial propagation in one motor column, and horizontal propagation between different motor columns.
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