Dual Stem Cell Therapy with Pharmacologically-Stimulated Mesenchymal Stem Cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659461
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Kyoto University
• Principal Investigator: MAEKAWA TAIRA 京都大学, 医学(系)研究科(研究院), 教授 (80229286)
• Co-Investigator(Kenkyū-buntansha): MIURA Yasuo 京都大学, 大学院医学研究科, 助教 (70605146) ; HIRAI Hideyo 京都大学, 大学院医学研究科, 助教 (50315933)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 間葉系幹細胞 / さい帯血 / 副甲状腺ホルモン

Research Abstract

This study has examined potential of mesenchymal stem cells that are stimulated by pharmacological agents for the application to hematopoietic stem cell transplantation. Parathyroid hormone improved the survival of lethally-irradiated mice in bone marrow transplantation and stimulated mesenchymal stem cells to enhance the expansion of hematopoietic cells. Mesenchymal stem cells can be isolated from an umbilical cord blood unit with small volume that is unavailable for umbilical cord blood transplantation. The isolated umbilical cord blood mesenchymal stem cells showed the proliferation and multi-differentiation capabilities that are equivalent to bone marrow mesenchymal stem cells.

Research Products

• [Journal Article] Parathyroid hormone enhances hematopoietic expansion via upregulation of cadherin-11 in bone marrow mesenchymal stromal cells (2014)
  • Author(s): Yao H, Miura Y, Yoshioka S, Miura M, Hayashi Y, Tamura A, Iwasa M, Sato A, Hishita T, Higashi Y, Kaneko H, Ashihara E, Ichinohe T, Hirai H, Maekawa T.
  • Journal Title: Stem Cells Volume: (in press)
  • DOI: 10.1002/stem.1701
  • Peer Reviewed
• [Journal Article] CCAAT/Enhancer-Binding ProteinβExpressed by Bone Marrow Mesenchymal Stromal Cells Regulates Early B-Cell Lymphopoiesis (2014)
  • Author(s): Yoshioka S, Miura Y, Yao H, Satake S, Hayashi Y, Tamura A, Hishita T, Ichinohe T, Hirai H, Takaori-Kondo A, Maekawa T.
  • Journal Title: Stem Cells Volume: 32 Pages: 730-740
  • DOI: 10.1002/stem.1555
  • Peer Reviewed
• [Journal Article] Chimerism of bone marrow mesenchymal stem/stromal cells in allogeneic hematopoietic cell transplantation : is it clinically relevant? (2013)
  • Author(s): Miura Y, Yoshioka S, Yao H, Takaori-Kondo A, Maekawa T, Ichinohe T.
  • Journal Title: Chimerism Volume: 4(3) Pages: 78-83
  • DOI: 10.4161/chim.25609
  • Peer Reviewed
• [Presentation] 間葉系幹細胞と副甲状腺ホルモンを応用した造血細胞の増幅 (2014)
  • Author(s): 三浦康生、平位秀世、前川平(他7名)
  • Organizer: 第36回日本造血細胞移植学会総会
  • Place of Presentation: 沖縄
  • Year and Date: 2014-03-08
• [Presentation] C/EBPβexpressed by bone marrow mesenchymal stromal cells is indispensable for precursor B-cell lymphopoiesis. American Society of Hematology (2013)
  • Author(s): Yoshioka S, Miura Y, Hirai H, Maekawa T(他7名)
  • Organizer: 55th Annual Meeting
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2013-12-09
• [Presentation] Direct Interaction with Bone Marrow Mesenchymal Stromal/Stem Cells Is Required for Hematopoietic Expansion by Parathyroid Hormone. American Society of Hematology (2013)
  • Author(s): Yao H , Miura Y, Hirai H, Maekawa T(他6名)
  • Organizer: 55th Annual Meeting
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2013-12-07
• [Presentation] Proliferation and differentiation of hematopoietic cells by osteogenic-induced bone marrow MSCs (2013)
  • Author(s): Yao H, Miura Y, Hirai H, Maekawa T(他7名)
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: Sapporo
  • Year and Date: 2013-10-12
• [Presentation] 接着法による効率的なさい帯血からの間葉系幹細胞分離に関する検討 (2013)
  • Author(s): 吉岡聡,三浦康生,平位秀世,前川平(他3名)
  • Organizer: 第61回日本輸血・細胞治療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-05-17
• [Presentation] Parathyroid hormone enhances expansion through CDH11 in human bone marrow mesenchymal stromal/stem cells (2013)
  • Author(s): Yao H, Miura Y, Hirai H, Maekawa T(他5名)
  • Organizer: AsiaCORD 2013, Scientific Symposium I-1"New Insights into Stem Cell Biology-Stem Cell Development & Differentiation"
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2013-04-19
• [Presentation] C/EBPβin mesenchymal stromal/stem cells regulates early B cell lymphopoiesis (2013)
  • Author(s): Yoshioka S, Miura Y, Hirai H, Maekawa T(他6名)
  • Organizer: AsiaCORD 2013, Scientific Symposium I-1"New Insights into Stem Cell Biology- Stem Cell Development & Differentiation"
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2013-04-19
• [Presentation] Mesenchymal stem cells skewed their phenotype toward the osteogenic lineage support the hematopoietic cell differentiation. American Society of Hematology (2012)
  • Author(s): Yao H, Miura Y, Hirai H, Maekawa T(他3名)
  • Organizer: 54th Annual Meeting
  • Place of Presentation: Atlanta, GA, USA
  • Year and Date: 20121208-11
• [Presentation] Expression of C/EBPβin bone marrow mesenchymal stem cells is mandatory for early-stage B cell lymphopoiesis. American Society of Hematology (2012)
  • Author(s): Yoshioka S, Miura Y, Hirai H, Maekawa T(他5名)
  • Organizer: 54th Annual Meeting
  • Place of Presentation: Atlanta, GA, USA
  • Year and Date: 20121208-11
• [Presentation] Parathyroid hormone stimulates mesenchymal stem cells to enhance expansion of hematopoietic cells (2012)
  • Author(s): Yao H, Miura Y, Hirai H, Maekawa T(他3名)
  • Organizer: The 74th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: Kyoto
  • Year and Date: 20121019-21
• [Presentation] Defective mesenchymal stem cells contribute to impaired B cell lymphopoiesis in C/EBPβdeficient mice (2012)
  • Author(s): Yoshioka S, Miura Y, Hirai H, Maekawa T(他5名)
  • Organizer: The 74th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: Kyoto
  • Year and Date: 20121019-21
• [Remarks]
  • URL: http://dtm.kuhp.kyoto-u.ac.jp