Improvement of Lymphocyte stimulation test.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659488
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Gunma University
• Principal Investigator: HIROKAZU Arakawa 群馬大学, 医学(系)研究科(研究院), 教授 (50272232)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: リンパ球刺激試験 / アレルギー / Ｔ細胞

Outline of Final Research Achievements

The lymphocyte stimulation test (LST) has been reported as a useful diagnostic for non-IgE mediated gastrointestinal food allergies. However, this method has some disadvantages including taking time, instability and relative a large amount of samples necessity. In this study, we propose the new method that overcomes the disadvantages of the LST. T cell receptor repertoire diversity was analyzed using next generation sequencing against the PBMC stimulated with milk antigen. After 24 hrs stimulation with antigen, some part of TCR clonotypes enriched slightly. We also tried to identify novel marker for T cell response to antigen. Several marker genes that the RNA expression level were changed by antigen stimulation were screened by gene expression array. The RNA expression level were quantified using qPCR and then compared to diagnosis by LST stimulation index. Some of these markers showed excellent discriminating accuracy (AUC0.79-0.91) by ROC analysis.

Free Research Field

小児科学