2013 Fiscal Year Annual Research Report
ヒトＥＳ／ｉＰＳ細胞由来の造血ストロマ細胞を用いた造血幹細胞の作出
| Project/Area Number |
24659496
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
中畑 龍俊 京都大学, iPS細胞研究所, 教授 (20110744)
|
|---|
| Keywords | ES細胞 / iPS細胞 / 造血幹細胞 / ストロマ細胞 / ニッチ |
|---|
| Research Abstract |
平成25年度は、前年度に同定した培養法の改変によってヒトES/iPS細胞由来造血支持ストロマの採取効率を改善した。さらにこれらの細胞を表面マーカーで分離し、どの分画が最も高い造血幹細胞（HSC）支持能を有するか、in vitroとin vivoの両面で測定した。具体的には、まず臍帯血CD34+細胞をそれぞれのストロマ分画上で2週間共培養した後にメチルセルロースコロニーアッセイ、およびNOGマウスへの移植実験を行った。
結果、我々の系で誘導されるVE-Cad陽性KDR陽性の血管内皮ストロマ分画が、従来から優れたHSC支持ストロマとして知られるマウスAGM細胞株とほぼ同等のHSC支持能を有することが示唆された。次に、ヒトES/iPSC由来造血幹前駆細胞に対する支持能を測定するため、同様の共培養実験を行った。in vitroでは極めて未熟な多分化性造血前駆細胞の増殖をAGM細胞同様に支持できることが示唆された。しかし、in vivoの移植実験で長期再構築能を有するES/iPSC由来HSCを検出するには至らなかった。
|
-
[Journal Article] Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis.2014
Author(s)
Morishima T, Watanabe KI, Niwa A, Hirai H, Saida S, Tanaka T, Kato I, Umeda K, Hiramatsu H, Saito MK, Matsubara K, Adachi S, Kobayashi M, Nakahata T, Heike T.
-
Journal Title
Haematologica
Volume: 99
Pages: 19-27
DOI
Peer Reviewed
-
[Journal Article] Excess reduction of anthracyclines results in inferior event-free survival in core binding factor acute myeloid leukemia in children; a report from the Japanese Pediatric Leukemia/Lymphoma Study Group (JPLSG).2014
Author(s)
Tomizawa D., Akio Tawa A., MD/PhD, Watanabe T., Saito A.M., Kudo K., Taga T., Iwamoto S., Shimada A., Terui K., Moritake H., Kinoshita A., Takahashi H., Nakayama H., Koh K., Kigasawa H., Kosaka Y., Miyachi H., Horibe K., Nakahata T., Adachi S.
-
Journal Title
Leukemia
Volume: in press
Pages: in press
DOI
Peer Reviewed
-
[Journal Article] PBSC collection from family donors in Japan: a prospective survey.2014
Author(s)
5. Kodera Y, Yamamoto K, Harada M, Morishima Y, Dohy H, Asano S, Ikeda Y, Nakahata T, Imamura M, Kawa K, Kato S, Tanimoto M, Kanda Y, Tanosaki R, Shiobara S, Kim SW, Nagafuji K, Hino M, Miyamura K, Suzuki R, Hamajima N, Fukushima M, Tamakoshi A
-
Journal Title
Bone Marrow Transplant
Volume: in press
Pages: in press
DOI
Peer Reviewed
-
[Journal Article] The clinical utility of genetic testing for t(8;16)(p11;p13) in congenital acute myeloid leukemia.2014
Author(s)
Daifu T., Kato I., Kozuki K., Umeda K., Hiramatsu H., Watanabe K., Kamiya I., Taki T., Nakahata T., Heike T., Adachi S.
-
Journal Title
J. Pediatr. Hematol/Onc.
Volume: in press
Pages: in press
Peer Reviewed
-
[Journal Article] Clinical characteristics of 15 children with juvenile myelomonocytic 1 leukemia who developed blast crisis: MDS Committee of Japanese Society of Pediatric Hematology/Oncology (JSPHO).2014
Author(s)
Honda Y., Tsuchida M.,, Zaike Y., Masunaga A., Yoshimi A., Kojima S., Ito M., Kikuchi A., Nakahata T., Manabe A.
-
Journal Title
Brit. J. Haematol.
Volume: in press
Pages: in press
DOI
Peer Reviewed
-
[Journal Article] Recipient seropositivity for adenovirus type 11 is a highly predictive factor for the development of AdV11-induced hemorrhagic cystitis after allogeneic hematopoietic SCT.2013
Author(s)
Nakazawa Y., Saito S., Yanagisawa R., Suzuki T., Toshiro Ito T., Ishida F., Muramatsu H., Matsumoto K., Kato K., Ishida H., Umeda K., Souichi Adachi S., Nakahata T., Koike K.
-
Journal Title
Bone Marrow Transplant.
Volume: 48
Pages: 737-739
DOI
Peer Reviewed
-
[Journal Article] Clonal selection in xenografted TAM recapitulates the evolutionary process of myeloid leukemia in Down syndrome.2013
Author(s)
Saida S, Watanabe KI, Sato-Otsubo A, Terui K, Yoshida K, Okuno Y, Toki T, Wang R, Shiraishi Y, Miyano S, Kato I, Morishima T, Fujino H, Umeda K, Hiramatsu H, Adachi S, Ito E, Ogawa S, Ito M, Nakahata T, Heike T.
-
Journal Title
Blood
Volume: 121
Pages: 4377-7387
DOI
Peer Reviewed
-
[Journal Article] Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell- free conditions.2013
Author(s)
Yanagimachi MD, Niwa A, Tanaka T, Ozaki F, Nishimoto S, Murata Y, Yasumi T, Ito J, Tomida S, Oshima K, Asaka I, Goto H, Heike T, Nakahata T, Saito MK
-
Journal Title
PLoS ONE
Volume: 8
Pages: e59243
DOI
Peer Reviewed
-
[Journal Article] Appropriate dose modification in induction therapy is essential for the treatment of infants with acute myeloid leukemia: a report from the Japanese Pediatric Leukemia/Lymphoma Study Group.2013
Author(s)
Tomizawa D., Tawa A., Watanabe T., Saito A.M., Kudo K., Taga T., Iwamoto S., Shimada A., Terui K.., Moritake H., Kinoshita A., Takahashi H., Nakayama H., Kiyokawa N., Isoyama K., Mizutani S., Hara J., Horibe K., Nakahata T., Souichi Adachi S.
-
Journal Title
Int. Hematol.
Volume: 98
Pages: 578-588
DOI
Peer Reviewed
-
-
-
[Presentation] Xenograft model of TAM reveals the evolutionary process of myeloid leukemia in Down syndrome.2013
Author(s)
Saida S., Watanabe K., Sato-Otsubo A., Terui K., Yoshida K., Okuno Y., Toki T., Wang R., Shiraishi Y., Miyano S., Kato I., Umeda K., Hiramatsu H., Fujino H., Adachi S., Nakahata T., Ito E., Ogawa S., Heike T.
Organizer
第75回日本血液学会学術集会
Place of Presentation
札幌市教育分化会館
Year and Date
20131011-20131013
-
-
-
-
-
-
-
-
-
-
