2013 Fiscal Year Final Research Report
Analyses of eccrine sweat reveal pathogenesis of atopic dermatitis
| Project/Area Number |
24659532
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Ehime University |
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Principal Investigator |
SAYAMA Koi 愛媛大学, 医学(系)研究科(研究院), 教授 (80187286)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | アトピー性皮膚炎 / 汗 / 角化細胞 / バリア異常 / IL-1 / IL-31 |
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| Research Abstract |
Eccrine sweat was collected from the arms of healthy volunteers after exercise, and levels of proinflammatory cytokines in the sweat were quantified by ELISA. We detected the presence of IL-1alpha, IL-1beta, and high levels of IL-31 in sweat samples. To investigate whether sweat activates keratinocytes, normal human keratinocytes were stimulated with concentrated sweat. Western blot analysis demonstrated the activation of NF-kB, ERK, and JNK signaling in sweat-stimulated keratinocytes. Real-time PCR using total RNA and ELISA analysis of supernatants showed the upregulation of IL-8 and IL-1beta by sweat. Furthermore, pretreatment with IL-1R antagonist blocked sweat-stimulated cytokine production and signal activation, indicating that bioactive IL-1 is a major factor in the activation of keratinocytes by sweat.
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