2013 Fiscal Year Final Research Report
Analyses of transcriptional factor Fra-1 function with a focus on EMT
| Project/Area Number |
24659583
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Chiba University |
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Principal Investigator |
HOSHINO Isamu 千葉大学, 医学部附属病院, 医員 (10400904)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Fra-1 / 食道扁平上皮癌 / HMGA1 / HMMR |
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| Research Abstract |
We have reported that Fra-1 was correlated with invasion and metastasis in esophageal squamous cell carcinoma(ESCC). Herein, we tried to clarify the mechanism of the Fra-1 expression in ESCC. We constructed Fra-1 knock down cells using siRNA in Fra-1 high expressed cells, TE10 and TE11. Fra-1 knock down induced reducing of cell proliferation, migration, and invasion potentials. Then, we explored the Fra-1 regulated genes using microarray and they were analyzed by BIOBASE Upstream Analysis. HMGA1 was a candidate which was regulated by Fra-1 and HMMR was detected as a gene which was regulated by HMGA1. Moreover, immunohistochemcal staining using clinical ESCC samples showed that HMMR was a significant prognostic factor in ESCC patients.
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