2013 Fiscal Year Final Research Report

Analyses of transcriptional factor Fra-1 function with a focus on EMT

Research Project

Project/Area Number	24659583
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	General surgery
Research Institution	Chiba University
Principal Investigator	HOSHINO Isamu 千葉大学, 医学部附属病院, 医員 (10400904)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	Fra-1 / 食道扁平上皮癌 / HMGA1 / HMMR
Research Abstract	We have reported that Fra-1 was correlated with invasion and metastasis in esophageal squamous cell carcinoma(ESCC). Herein, we tried to clarify the mechanism of the Fra-1 expression in ESCC. We constructed Fra-1 knock down cells using siRNA in Fra-1 high expressed cells, TE10 and TE11. Fra-1 knock down induced reducing of cell proliferation, migration, and invasion potentials. Then, we explored the Fra-1 regulated genes using microarray and they were analyzed by BIOBASE Upstream Analysis. HMGA1 was a candidate which was regulated by Fra-1 and HMMR was detected as a gene which was regulated by HMGA1. Moreover, immunohistochemcal staining using clinical ESCC samples showed that HMMR was a significant prognostic factor in ESCC patients.

(3 results)