2013 Fiscal Year Final Research Report
Elucidation of liver carcinogenesis mechanism by epigenetic reprogramming.
| Project/Area Number |
24659593
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 癌 / エピジェネティクス |
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| Research Abstract |
As a result of introduction of the 4 factors to the HCC cell lines, colonies of iPS cell-like is formed after about 2 weeks. The established clones were investigated by morphological observation, gene expression analysis and the transplantation into immunodeficient mice. Expression of hepatocyte marker HNF4 in comparison with HCC cell lines were decreased. And increased expression of Sox2, undifferentiated marker, was observed. However, the expression of undifferentiated markers were lower compared with iPS cells. To verify the presence or absence of teratoma formation capability, clones were transplanted into the testes of immunodeficient mice (NOD / scid). However no teratoma formation was observed from clones that were established in this study.
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Research Products
(29 results)
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[Journal Article] The CD133+CD44+ Precancerous Subpopulation of Oval Cells is a Therapeutic Target for Hepatocellular2014
Author(s)
Zheng YW, Tsuchida T, Shimao T, Li B, Takebe T, Zhang RR, Sakurai Y, Ueno Y, Sekine K, Ishibashi N, Imajima M, Tanaka T, Taniguchi H
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Journal Title
Carcinoma Stem Cells Dev
Volume: (in press)
Peer Reviewed
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[Journal Article] Ring1B promotes hepatic stem/ progenitor cell expansion via simultaneous suppression of Cdkn1a and Cdkn2a2014
Author(s)
Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng YW,Takebe T, Isono KI, Koseki H, Taniguchi H
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Evidence of a sophisticatedly heterogeneous population of human umbilical vein endothelial cells2014
Author(s)
Zheng YW, Nie YZ, Tsuchida T, Zhang RR, Aoki K, Sekine K, Ogawa M, Takebe T, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
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Journal Title
Transplant Proc
Volume: 46(4)
Pages: 1251-3
DOI
Peer Reviewed
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[Journal Article] Vascularized and functional human liver tissue from an induced pluripotent stem cell-derived organ bud transplant2013
Author(s)
Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zhang RR, Ueno Y, Zheng YW, Koike N, Aoyama S, Adachi Y, Taniguchi H
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Journal Title
Nature
Volume: 499(7459)
Pages: 481-484
DOI
Peer Reviewed
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[Journal Article] Vitamin D Receptor in osteoblasts Is a negative regulator of bone mass control2013
Author(s)
Yamamoto Y, Yoshizawa T, Fukuda T, Shirode-Fukuda Y, Yu T, Sekine K, Sato T, Kawano H, Aihara KI, Nakamichi Y, Watanabe T, Shindo M, Inoue K, Inoue E, Tsuji N, Hoshino M, Karsenty G, Metzger D, Chambon P, Kato S, Imai Y
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Journal Title
Endocrinology
Volume: 154(3)
Pages: 1008-1020
DOI
Peer Reviewed
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[Journal Article] Inhibition of Stabilin-2 elevates the circulating hyaluronic acid level and prevents tumor metastasis2012
Author(s)
Hirose Y, Saijou E, Sugano Y, Takeshita F, Nishimura S, Nonaka H, Chen YR, Sekine K, Kido T, Nakamura T, Kato S, Kanke T, Nakamura K, Nagai R, Ochiya T, Miyajima A
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Journal Title
Proc Natl Acad Sci U S A
Volume: 109 (11)
Pages: 4263-4268
DOI
Peer Reviewed
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[Presentation] Targeted proteomic absolute quantification on transporters of human pancreatic cancer cells with gemcitabine-resistance2013
Author(s)
Ueno Y, Hoshino S, Tsuchida N, Nakata S, Uchida Y, Sekine K, Zheng YW, Kurata M, Morinaga S, Miyagi Y, Yokose T, Endo I, Terasaki T, Hirano H, Taniguchi H
Organizer
HUPO 12th annual world congress
Place of Presentation
Yokohama, Japan
Year and Date
20130914-18
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