Somatic cell to CMs by miRNA

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659594
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: GOJO Satoshi 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ncRNA / miRNA / 再生医療 / 心筋分化誘導

Outline of Final Research Achievements

Non-coding RNAs (ncRNAs) are involved in the regulation of most biological processes such as cancer, embryonic development, and cellular reprogramming. However, little is known about their roles in iPSC reprogramming. To elucidate the changing of non-coding RNAs during iPSC reprogramming, human fibroblasts TIG1 were infected with OCT4, SOX2, KLF4, and c-MYC (OSKM). Total RNAs from OSKM-TIG1 were analyzed for the ncRNAs expression.Expression of Y-RNA1 transiently increased during early stage (Day 0 to 9 after the transduction), and the localization was cellular cytoplasm. To evaluate the effect of the selected ncRNA, Y-RNA1, we assessed the efficacy of iPSCs reprogramming by the knockdown and over-expression experiments. The siRNA for Y-RNA1 was introduced to TIG1, where Y-RNA1 expression lessens to less than 10%, compared with conventional iPSC reprogramming. Our data demonstrate that Y-RNA1 is essential role for iPSC reprogramming, and works at the early stage of the process.

Free Research Field

再生医療