2013 Fiscal Year Final Research Report
Purification and culture of circulating tumor cells in gastrointestinal cancer patients
| Project/Area Number |
24659607
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
NISHIMURA Junichi 大阪大学, 医学(系)研究科(研究院), 助教 (20379209)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 遊離癌細胞 / miRNA |
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| Research Abstract |
We sorted circulating cells in blood of colorectal patients using Percoll treatment and FACS analysis. EpCAM+CD44+ cells were all CD45 positive. CD45-EpCAM+ cells were detected 10 cells of 4X105 cells. However, these cells were detected also in healthy volunteer, indicating that all these cells were not circulating cells originated from cancer cells. Next, we analyzed blood circulating miRNA in colorectal patients. miRNA expression of preoperative and postoperative blood samples were analyzed using microarray analysis. Four miRNAs were detected to be a colorectal cancer specific miRNA.
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