The possible mechanism on homing effect of adipose-derived stem cells to the injured liver

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659611
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: The University of Tokushima
• Principal Investigator: SHIMADA Mitsuo 徳島大学, ヘルスバイオサイエンス研究部, 教授 (10216070)
• Co-Investigator(Kenkyū-buntansha): MORINE Yuji 徳島大学, 大学院ヘルスバイオサイエンス研究部, 講師 (60398021) ; IMURA Satoru 徳島大学, 病院, 特任教授 (90380021) ; UTSUNOMIYA Toru 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准講師 (30304801)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 肝不全 / 幹細胞 / homing効果 / 遺伝子解析

Outline of Final Research Achievements

This study focused on the regulation of homing effect of systemically transplanted human adipose-derived stem cells(ADRCs) into the injured liver. ADRCs indicated the reduction of liver injury with 70% hepatectomy(Hx) and ischemia-reperfusion(I/R) in mouse model.ADRCs were traced by in vivo imaging for 24h,and found in various organs immediately following transplantation and almost accumulated in remnant liver or spleen at 6 h after transplantation. Also,ADRCs were histologically revealed in the harvested liver.70%Hx and I/R injury significantly enhanced SDF-1 expressions regardless of ADRCs transplantation,and only ADRC transplantation increased CXCR-4 expressions. The predominant SDF-1 positive cells in the liver were equally identified in parenchymal and nonparenchymal cells at 6h,but shifted to non-parenchymal cells at 24 h after transplantation. Systemically transplanted ADRCs homed to the injured liver after transplantation,possibly based on the mechanisms of SDF-1/CXCR-4 axis.

Free Research Field

消化器外科