Prediction of Response to Molecular-targeting agents in colorectal cancer By Gene expression Analysis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659620
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: The University of Tokyo
• Principal Investigator: WATANABE TOSHIAKI 東京大学, 医学部附属病院, 教授 (80210920)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 大腸癌 / DNAマイクロアレイ / 化学療法 / 遺伝子発現

Research Abstract

Based on the gene expression of colorectal cancers, we aimed to build a predictive model for response to chemotherapy based on molecular target agent (bevacizumab). Using 41 samples, we examined gene expression by microarray, then validated them by RT-PCR method. We finally selected 3- and 2-gene sets and made 2-gene and 3-gene predictive models. The accuracy rate of prediction was 73.2 %(3-gene model) and 75.6%(2-gene model). We also examined the correlations of these genes with individual drug responses on tumor cell lines deposited on public database.

Research Products

• [Journal Article] Prediction of response to preoperative chemoradiotherapy in rectal cancer by using reverse transcriptase polymerase chain reaction analysis of four genes (2014)
  • Author(s): Watanabe T, Kobunai T, Akiyoshi T, Matsuda K, Ishihara S, Nozawa K
  • Journal Title: Dis Colon Rectum Volume: 57(1) Pages: 23-31
  • Peer Reviewed
• [Journal Article] Salvage Gastrectomy After Intravenous and Intraperitoneal Paclitaxel (PTX) Administration with Oral S-1 for Peritoneal Dissemination of Advanced Gastric Cancer with Malignant Ascites (2013)
  • Author(s): Kitayama J, Ishigami H, Yamaguchi H, Yamashita H, Emoto S, Kaisaki S, Watanabe T
  • Journal Title: Annals of Surgical Volume: 21(2) Pages: 539-46
  • Peer Reviewed
• [Journal Article] A phase 2 trial of intravenous and intraperitoneal paclitaxel combined with S-1 for treatment of gastric cancer with macroscopic peritoneal metastasis (2013)
  • Author(s): Yamaguchi H, Kitayama J, Ishigami H, Emoto S, Yamashita H, Watanabe T
  • Journal Title: Cancer Volume: 15;119(18) Pages: 3354-8
  • Peer Reviewed
• [Journal Article] KRAS Mutational Status in Japanese Patients with Colorectal Cancer : Results from a Nationwide, Multicenter, Cross-sectional Study (2013)
  • Author(s): Watanabe T, Yoshino T, Uetake H, Yamazaki K, Ishiguro M, Kurokawa T, Saijo N, Ohashi Y, Sugihara K
  • Journal Title: Japanese Journal of Clinical Oncology Volume: 43(7) Pages: 706-12
  • Peer Reviewed
• [Journal Article] Recent approaches to identifying biomarkers for high-risk stage II colon cancer (2012)
  • Author(s): Akiyoshi T, Kobunai T, Watanabe T
  • Journal Title: Surg Today Volume: 42(11) Pages: 1037-45
  • Peer Reviewed
• [Journal Article] Predicting the response to preoperative radiation or chemoradiation by a microarray analysis of the gene expression profiles in rectal cancer (2012)
  • Author(s): Akiyoshi T, Kobunai T, Watanabe T
  • Journal Title: Surg Today Volume: 42(8) Pages: 713-9
  • Peer Reviewed
• [Journal Article] Chromosomal instability (CIN) phenotype, CIN high or CIN low, predicts survival for colorectal cancer (2012)
  • Author(s): Watanabe T, Kobunai T, Yamamoto Y, Matsuda K, Ishihara S, Nozawa K, Yamada H, Hayama T, Inoue E, Tamura J, Iinuma H, Akiyoshi T, Muto T
  • Journal Title: J Clin Oncol Volume: 20;30(18) Pages: 2256-64
  • Peer Reviewed