2013 Fiscal Year Final Research Report
Radio-immune fusion therapy for cancer; ER stress loading with radiation and celecoxib
| Project/Area Number |
24659644
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
TSUBOI Koji 筑波大学, 医学医療系, 教授 (90188615)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Tadao 早稲田大学, 理工学術院, 客員教授 (90160580)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 悪性脳腫瘍 / 放射線 / 腫瘍免疫 / ERストレス / Celecoxib |
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| Research Abstract |
We found that the anti-inflammatory drug celecoxib significantly enhanced the radiosensitivity of mouse glioblastoma cells GL261 by loading ER stress. Based on this, we established GL261 cells constitutively expressing monomeric Kusabira-Orange (GL261-mKO) that were used to generate a mouse thigh subcutaneous tumor model in syngenic albino C57BL/6 mice, in which tumor size can be monitored by an in vivo imaging system. In experiments with local X-ray irradiation (IR) to tumors in this system, the cure rate was significantly higher with the concomitant oral administration of 5mg/kg of celecoxib as compared to IR alone. Furthermore, the same tumor cells that were re-challenged to the brain in the cured mice were all rejected, while they grew rapidly in mice that showed tumor regrowth after IR. ELISPOT assay demonstrated that IFN-gamma level was significantly elevated in mice that rejected re-challenged tumor cells, indicating that anti-tumor cellular immunity was established.
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[Journal Article] A phase I study on combined therapy with proton-beam radiotherapy and in situ tumor vaccination for locally advanced recurrent hepatocellular carcinoma2013
Author(s)
Abei M, Okumura T, Fukuda K, Hashimoto T, Araki M, Ishige K, Hyodo I, Kanemoto A, Numajiri H, Mizumoto M, Sakae T, Sakurai H, Zenkoh J, Ariungerel G, Sogo Y, Ito A, Ohno T, Tsuboi K
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Journal Title
Radiat Oncol
Volume: 8(1)
Pages: 239
DOI
Peer Reviewed
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