2013 Fiscal Year Final Research Report
Novel regulator of gene expression in DRG in a neuropathic pain
| Project/Area Number |
24659688
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 脊髄神経節 / 遺伝子発現 / Artemin / TRPV1 / TRPA1 |
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| Research Abstract |
We found the following findings. 1. Artemin increases locally in skin over long periods of time, whereas NGF shows transient increases after peripheral inflammation. 2. Artemin increases in peripheral sciatic nerve following to the Wallerian degeneration after nerve injury. 3. Repeated artemin injections changed the gene expressions of TRPV1 and TRPA1 in DRG neurons. 4. The co-localization between TRPV1/A1 and GFRalpha3 was higher than that between TRPV1/A1 and TrkA. 5. Continuous artemin injection into the plantar surface caused mechanical and heat hyperalgesia. 6. Artemin increases TRPV1/A1 expression and Ca activity in primary cultured DRG neurons. 7. TRPV1/A1 expression was regulated by artemin through p38 phosphorylation in primary afferent.
We conclude that peripheral tissue-derived artemin synthesis is important regulator of TRPV1/A1 expression in DRG neurons following peripheral inflammation and nerve injury.
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Research Products
(24 results)
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[Journal Article] Potentiation of the P2X3 ATP receptor by PAR-2 in rat DRG neurons, through protein kinase-dependent mechanisms, contributes to inflammatory pain2012
Author(s)
Wang S, Dai Y, Kobayashi K, Zhu W, Kogure Y, Yamanaka H, Wan Y, Zhang W, Noguchi K
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Journal Title
Eur J Neurosci
Volume: 36
Pages: 2293-301
DOI
Peer Reviewed
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