Utility value of blastocyst biopsy for preimplantation genetic diagnosis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659739
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: TOMIYAMA Ryoko 日本医科大学, 医学部, アシスタント・スタッフ (40409214)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 胚盤胞生検 / 分裂期

Research Abstract

Embryo biopsy intended for preimplantation genetic diagnosis (PGD) is generally performed on cleavage stage, but it tend to be harmful for embryo viability. Trophectoderm biopsy from blastocyst is seem to be less invasive, since inner cell mass is remain intact after biopsy. In this study it could be easily biopsied several number of trophectoderm cells and all of blastocysts expanded again after biopsy. Some blastocyst cells treated by Colcemid were significantly interrupted mitosis, which drug efficacy was confirmed by Hoechst and Propidium iodide staining. On the other hand, fluorescent signals of whole chromosome painting probes were strongly detectable as for lymphocyte FISH. Trophectoderm biopsiy followed by examination using mitotic stage cells was likely to useful for PGD.