photoreceptor cell sheet derived from mouse iPS cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659769
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: UEDA Yuji 聖マリアンナ医科大学, 医学部, 講師 (00223470)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Noboru 聖マリアンナ医科大学, 医学部, 教授 (40235982) ; ARIMITSU Nagisa 聖マリアンナ医科大学, 医学部, 助教 (40408688)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: iPS細胞 / 網膜 / 視細胞 / ロドプシン

Outline of Final Research Achievements

The retinal tissue is derived from the central nervous system and does not have the regeneration ability. Thus once damaged, regeneration of retina is almost impossible. We generated retinal progenitor cell lines from mouse iPS cells transfected with pax6 cDNA, and by using the cloned retinal progenitors we made retinal cell sheet including rhodopsin positive photoreceptor cells. They made massive synaptic connection among them and the sheet looked resembling naïve retinal neuron layers. It could be clinically applicable for patients with damaged retina.

Free Research Field

再生医学