A novel strategy for heat stroke: targeted therapy for free nucleotides

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659796
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Osaka University
• Principal Investigator: OGURA Hiroshi 大阪大学, 医学(系)研究科(研究院), 准教授 (70301265)
• Co-Investigator(Kenkyū-buntansha): SHIMAZU Takeshi 大阪大学, 医学系研究科, 教授 (50196474) ; KUWAGATA Yasuyuki 大阪大学, 医学系研究科, 招聘教授 (50273678) ; MATSUMOTO Naoya 大阪大学, 医学部附属病院, 招聘教員 (50359808)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 熱中症 / 核酸 / 遊離 / 血管内皮 / 臓器障害 / DNase / RNase

Outline of Final Research Achievements

The number of patients with heat stroke is globally increasing, and novel therapeutic interventions to prevent the progress of organ damages are urgently required. The objectives of this study were to evaluate the pathophysiology after heat stroke and to examine the effects of DNase and RNase as anti-DAMPs (damage associated molecular patterns) therapies in a rat model. As results, there was no significant difference in mortality between DNase or RNase group and the control group. The serum concentrations of cytokines significantly increased following heat stroke, and there was no significant difference in these variables between DNase or RNase group and the control group. The enhanced vascular permeability in guts, brain or lung, evaluated by Evans blue technique was not attenuated by the administration of DNase or RNase following heat stroke. These results indicate that DNase and RNase as anti-DAMPs therapies did not exert beneficial effects in our heat stroke model.

Free Research Field

救急医学