2013 Fiscal Year Final Research Report
Circulating tumor-derived mutant mitochondrial DNA: a predictive biomarker of clinical prognoses in human squamous cell carcinoma
| Project/Area Number |
24659885
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Chiba University |
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Principal Investigator |
TANZAWA HIDEKI 千葉大学, 医学(系)研究科(研究院), 教授 (50236775)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Hirofumi 千葉大学, 医学部附属病院, 助教 (10595995)
KOUZU Yukinao 千葉大学, 大学院医学研究院, 助教 (70400942)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | mtDNA / 口腔癌 / qPCR-HRMA / 予後判定 |
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| Research Abstract |
In a variety of malignant tumors, it is difficult to detect the circulating tumor-derived molecular targets. But ,in murine xenografting human oral cancer cells, human mtDNAs could be quantitatively detected from organs and blood samples. In the present study, we used this mtDNA for patients with oral cancer, and analyzed all mitochondrial loop domains. As a result, we found that the oral cancer derived cell lines had mutation at 5 regions in mtDNA. mtDNA from normal and tumorous tissues and serum mtDNA obtained preoperatively and 4 weeks postoperatively from 61 subjects was examined at these five different regios. All patients with a poor prognosis (recurrence and/or distant metastasis cases, r/m+) had significantly (P<0.05) higher amounts of mutant mtDNAs in the tumoral tissues. These results suggested that this approach may have useful diagnosis for predictor of the prognosis.
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